| Title: | Physical and functional interactions between ZIP kinase and UbcH5 |
| Other Titles: | Interactions between ZIPK with UbcH5 |
| Authors: | Ohbayashi, Norihiko Browse this author |
| Okada, Katsuya Browse this author |
| Kawakami, Shiho Browse this author |
| Togi, Sumihito Browse this author |
| Sato, Noriko Browse this author |
| Ikeda, Osamu Browse this author |
| Kamitani, Shinya Browse this author |
| Muromoto, Ryuta Browse this author |
| Sekine, Yuichi Browse this author |
| Kawai, Taro Browse this author |
| Akira, Shizuo Browse this author |
| Matsuda, Tadashi Browse this author →KAKEN DB |
| Keywords: | ZIPK |
| UbcH5 |
| ubiquitination |
| PML nuclear body |
| Issue Date: | 8-Aug-2008 |
| Publisher: | Academic Press |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 372 |
| Issue: | 4 |
| Start Page: | 708 |
| End Page: | 712 |
| Publisher DOI: | 10.1016/j.bbrc.2008.05.113 |
| PMID: | 18515077 |
| Abstract: | Zipper-interacting protein kinase (ZIPK) is a widely expressed serine/threonine kinase that has been implicated in cell death and transcriptional regulation, but its mechanism of regulation remains unknown. In our previous study, we showed that leukemia inhibitory factor stimulated threonine-265 phosphorylation of ZIPK, thereby leading to phosphorylation and activation of signal transducer and activator of transcription 3. Here, we identified UbcH5c as a novel ZIPK-binding partner by yeast two-hybrid screening. Importantly, we found that UbcH5c induced ubiquitination of ZIPK. Small-interfering RNA-mediated reduction of endogenous UbcH5 expression decreased ZIPK ubiquitination. Furthermore, coexpression of UbcH5c with ZIPK influenced promyelocytic leukemia protein nuclear body (PML-NB) formation. These results suggest that UbcH5 regulates ZIPK accumulation in PML-NBs by interacting with ZIPK and stimulating its ubiquitination. |
| Relation: | http://www.sciencedirect.com/science/journal/0006291X |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/33898 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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