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Genetic and antigenic analyses of a Puumala virus isolate as a potential vaccine strain

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.56.3.151

Title: Genetic and antigenic analyses of a Puumala virus isolate as a potential vaccine strain
Authors: Abu Daud, Nur Hardy Browse this author
Kariwa, Hiroaki Browse this author
Tkachenko, Evgeniy Browse this author
Dzagurnova, Tamara Browse this author
Medvedkina, Olga Browse this author
Tkachenko, Peter Browse this author
Ishizuka, Mariko Browse this author
Seto, Takahiro Browse this author
Miyashita, Daisuke Browse this author
Sanada, Takahiro Browse this author
Nakauchi, Mina Browse this author
Yoshii, Kentaro Browse this author
Maeda, Akihiko Browse this author
Yoshimatsu, Kumiko Browse this author
Arikawa, Jiro Browse this author
Takashima, Ikuo Browse this author
Keywords: hantavirus
hemorrhagic fever with renal syndrome
Puumala virus
vaccine
Issue Date: Nov-2008
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 56
Issue: 3
Start Page: 151
End Page: 165
Abstract: Puumala virus (PUUV), a causative agent of hemorrhagic fever with renal syndrome (HFRS), is prevalent in Europe and European Russia. No vaccine has been developed for PUUVassociated HFRS, primarily because of the low viral yield in cultured cells. A PUUV strain known as DTK/Ufa-97 was isolated in Russia and adapted for growth in Vero E6 cells maintained in serum-free medium. The DTK/Ufa-97 strain produced a higher viral titer in serumfree medium, suggesting that it may prove useful in the development of an HFRS vaccine.When PUUV-infected Vero E6 cells were grown in serum-free medium, the DTK/Ufa-97 strain yielded more copies of intracellular viral RNA and a higher viral titer in the culture fluid than did the Sotkamo strain. Phylogenetic analysis revealed that PUUVs can be classified into multiple lineages according to geographical origin, and that the DTK/Ufa-97 strain is a member of the Bashkiria-Saratov lineage. The deduced amino acid sequences of the small, medium, and large segments of the DTK/Ufa-97 strain were 99.2% to 100%, 99.3% to 99.8%, and 99.8% identical, respectively, to those of the Bashkirian PUUV strains and 96.9%, 92.6%, and 97.4% identical, respectively, to those of the Sotkamo strain, indicating that the PUUVs are genetically diverse. However, DTK/Ufa-97 and other strains of PUUV exhibited similar patterns of binding to a panel of monoclonal antibodies against Hantaan virus. In addition, diluted antisera (i.e., ranging from 1:160 to 1:640) specific to three strains of PUUV neutralized both homologous and heterologous viruses. These results suggest that the DTK/Ufa-97 strain is capable of extensive growth and is antigenically similar to genetically distant strains of PUUV.
Type: bulletin
URI: http://hdl.handle.net/2115/35115
Appears in Collections:Volume 56, Number 3

 

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