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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
初代ラット大脳皮質由来神経細胞の凍結保存条件に及ぼす凍結速度依存性
| Title: | 初代ラット大脳皮質由来神経細胞の凍結保存条件に及ぼす凍結速度依存性 |
| Other Titles: | Effects of Cooling Rate on Cryopreservation Conditions of Primary Rat Cortical Cells |
| Authors: | 内田, 努1 Browse this author →KAKEN DB | | 本村, 寿太郎2 Browse this author | | 永山, 昌史3 Browse this author →KAKEN DB | | 郷原, 一寿4 Browse this author →KAKEN DB |
| Authors(alt): | Uchida, Tsutomu1 | | Motomura, Jutaro2 | | Nagayama, Masafumi3 | | Gohara, Kazutoshi4 |
| Keywords: | Neuron | | Cryopreservation | | Cooling rate | | Action potential | | 神経細胞 | | 凍結保存 | | 凍結速度 | | 活動電位 |
| Issue Date: | 30-Dec-2007 |
| Publisher: | 低温生物工学会 |
| Journal Title: | 低温生物工学会誌 |
| Journal Title(alt): | Cryobiology and Cryotechnology |
| Volume: | 53 |
| Issue: | 2 |
| Start Page: | 161 |
| End Page: | 166 |
| Abstract: | Developments of cryopreservation techniques on primary rat cortical cells were carried out to observe the effect of cooling rates. Primary rat cortical cells were dispersed in the cryopreserving medium including 5 vol% dimethylsulfoxide, and then they were frozen by various cooling rates ranged from -3 K/min to -150 K/min. The survival ratio was estimated qualitatively by using the phase-contrast microscope. The feasibility of the neural activity was verified by measuring its spontaneous action potential with a multi-electrode array system. Based on these characterizations, the lower cooling rate of the dispersed sample was more feasible for the cryopreservation of the primary rat cortical cells. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/36104 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 内田 努
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