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Detection of changes in the nuclear phase and evaluation of male germ units by flow cytometry during in vitro pollen tube growth in Alstroemeria aurea

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Title: Detection of changes in the nuclear phase and evaluation of male germ units by flow cytometry during in vitro pollen tube growth in Alstroemeria aurea
Authors: Hirano, Tomonari Browse this author
Hoshino, Yoichiro Browse this author →KAKEN DB
Keywords: flow cytometry
generative cell
male germ unit
pollen protoplast
sperm cell
Issue Date: Mar-2009
Publisher: Botanical Society of Japan
Journal Title: Journal of Plant Research
Volume: 122
Issue: 2
Start Page: 225
End Page: 234
Publisher DOI: 10.1007/s10265-008-0208-2
PMID: 19151916
Abstract: This study aimed to analyze male gamete behavior from mature pollen to pollen tube growth in the bicellular pollen species Alstroemeria aurea. For mature pollen, pollen protoplasts were examined using flow cytometry. The protoplasts showed two peaks of DNA content at 1C and 1.90C. Flow cytometry of pollen tubes at different developmental stages that were cultured in vitro revealed changes in the nuclear phase at 9 h and 18 h after culture. Sperm cell formation occurred at 6–9 h after culture, indicating that the first change was due to the division of the generative cells into sperm cells. After sperm cell formation, the number of vegetative nucleus associations with sperm cell showed a tendency to increase. The association was suggested as male germ unit. When sperm cells, vegetative nuclei, and partial male germ units were separately collected from pollen tubes cultured for 18 h and analyzed using a flow cytometer, the sperm cells and vegetative nuclei contained 1C DNA, while the DNA content of partial male germ units was counted as 2C. Therefore, the second change in the nuclear phase, which resulted in an increase in 2C nuclei, was possibly related to the formation of male germ units.
Rights: The original publication is available at www.springerllink.com.
Type: article (author version)
URI: http://hdl.handle.net/2115/36650
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 星野 洋一郎

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