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Plaque Assay of Oncorhynchus masou Virus (OMV)

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Title: Plaque Assay of Oncorhynchus masou Virus (OMV)
Authors: Kamei, Yuto Browse this author
Yoshimizu, Mamoru Browse this author →KAKEN DB
Kimura, Takahisa Browse this author →KAKEN DB
Issue Date: Sep-1987
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 22
Issue: 3
Start Page: 147
End Page: 152
Abstract: Plaque assays for salmonid virus, Oncorhynchus masou virus (OMV), were examined and compared with infectious haematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV), combining various fish cell-lines and three overlays. OMV produced the plaques only in chinook salmon embryo (CHSE-214) cells and rainbow trout gonad (RTG-2) cells, irrespective of overlays used, but plaque formation of IHNV and IPNV differed in different cell-lines according to the overlays used. The plaquing procedure using CHSE-214 cells gave a ten-fold higher number of plaques than RTG-2 cells. Among three overlays of methylcellulose, gum tragacanth, and agarose, methylcellulose overlay facilitated OMV plaque formation, and the plaques were most clearcut and easily enumerated. On the whole, the combination of methylcellulose overlay and CHSE-214 cell-line was the most suitable for plaque assay of OMV as well as IHNV and IPNV.
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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