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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Plaque Assay of Oncorhynchus masou Virus (OMV)
| Title: | Plaque Assay of Oncorhynchus masou Virus (OMV) |
| Authors: | Kamei, Yuto Browse this author | | Yoshimizu, Mamoru Browse this author →KAKEN DB | | Kimura, Takahisa Browse this author →KAKEN DB |
| Issue Date: | Sep-1987 |
| Publisher: | 日本魚病学会 |
| Journal Title: | 魚病研究 |
| Journal Title(alt): | Fish pathology |
| Volume: | 22 |
| Issue: | 3 |
| Start Page: | 147 |
| End Page: | 152 |
| Abstract: | Plaque assays for salmonid virus, Oncorhynchus masou virus (OMV), were examined and compared with infectious haematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV), combining various fish cell-lines and three overlays. OMV produced the plaques only in chinook salmon embryo (CHSE-214) cells and rainbow trout gonad (RTG-2) cells, irrespective of overlays used, but plaque formation of IHNV and IPNV differed in different cell-lines according to the overlays used. The plaquing procedure using CHSE-214 cells gave a ten-fold higher number of plaques than RTG-2 cells. Among three overlays of methylcellulose, gum tragacanth, and agarose, methylcellulose overlay facilitated OMV plaque formation, and the plaques were most clearcut and easily enumerated. On the whole, the combination of methylcellulose overlay and CHSE-214 cell-line was the most suitable for plaque assay of OMV as well as IHNV and IPNV. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/38326 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 吉水 守
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