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Comparative Growth Response of Fish Cell Lines in Different Media, Temperatures, and Sodium Chloride Concentrations

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Title: Comparative Growth Response of Fish Cell Lines in Different Media, Temperatures, and Sodium Chloride Concentrations
Authors: Fernandez, Roselyn D. Browse this author
Yoshimizu, Mamoru Browse this author →KAKEN DB
Ezura, Yoshio Browse this author
Kimura, Takahisa Browse this author
Issue Date: Mar-1993
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish pathology
Volume: 28
Issue: 1
Start Page: 27
End Page: 34
Abstract: Growth responses to three different media, temperatures, and sodium chloride concentrations in the media were determined for 13 salmonid and 14 non-salmonid fish cell lines. Most cell lines showed better growth in Eagle's MEM than in Medium 199 or in Leivobitz L-15 medium. Nine salmonid cell lines grew well in the normal sodium bicarbonate buffer in Eagle's MEM, while 11 non-salmonid cell lines grew better in Eagle's MEM buffered with either HEPES-bicarbonate or Tris-bicarbonate. Optimum temperature for growth ranged from 15 to 20℃ for almost all salmonid cells and 20 to 30℃ for non-salmonid cells. Most of the cell lines showed highest growth in commercial medium preparations with the lowest concentrration of sodium chloride (0.116M) examined. However, three of the six cell lines derived from rainbow trout, Oncorhynchus mykiss, and cell lines from eels, Anguilla japonica, showed optimum growth response at a higher sodium chloride concentration of 0.171M in the medium.
Type: article
URI: http://hdl.handle.net/2115/38339
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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