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STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation
Title: | STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation |
Other Titles: | Brk phosphorylates and regulates STAP-2 functions |
Authors: | Ikeda, Osamu Browse this author | Miyasaka, Yuto Browse this author | Sekine, Yuichi Browse this author | Mizushima, Akihiro Browse this author | Muromoto, Ryuta Browse this author | Nanbo, Asuka Browse this author | Yoshimura, Akihiko Browse this author | Matsuda, Tadashi Browse this author →KAKEN DB |
Keywords: | Brk | STAP-2 | phosphorylation | STAT3 | transcription |
Issue Date: | 19-Jun-2009 |
Publisher: | Academic Press |
Journal Title: | Biochemical and Biophysical Research Communications |
Volume: | 384 |
Issue: | 1 |
Start Page: | 71 |
End Page: | 75 |
Publisher DOI: | 10.1016/j.bbrc.2009.04.076 |
PMID: | 19393627 |
Abstract: | Signal transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein that contains Pleckstrin and Src homology 2 (SH2)-like domains as well as a YXXQ motif in its C-terminal region. STAP-2 is also known as breast tumor kinase (Brk) substrate (BKS). Our previous studies revealed that STAP-2 binds to signal transducer and activator of transcription 3 (STAT3) and STAT5, and regulates the signaling pathways downstream of them. In the present study, we identified tyrosine-250 (Tyr250) in STAP-2 as a major site of phosphorylation by Brk, using a series of STAP-2 YF mutants and anti-phospho-STAP-2 Tyr250 antibody. Furthermore, overexpression of the STAP-2 Y250F mutant protein affected Brk-mediated STAT3 activation. Importantly, small-interfering RNA-mediated reduction of endogenous STAP-2 expression decreased Brk-mediated STAT3 activation. Taken together, our findings demonstrate that STAP-2 is phosphorylated at Tyr250 by Brk, and plays an important role in Brk-mediated STAT3 activation. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/38456 |
Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 正
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