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The protein content of an adaptor protein, STAP-2 is controlled by E3 ubiquitin ligase Cbl
| Title: | The protein content of an adaptor protein, STAP-2 is controlled by E3 ubiquitin ligase Cbl |
| Other Titles: | Cbl controls STAP-2 activity through protein degradation |
| Authors: | Sekine, Yuichi Browse this author | | Yamamoto, Chikako Browse this author | | Ikeda, Osamu Browse this author | | Muromoto, Ryuta Browse this author | | Nanbo, Asuka Browse this author | | Oritani, Kenji Browse this author | | Yoshimura, Akihiko Browse this author | | Matsuda, Tadashi Browse this author →KAKEN DB |
| Keywords: | STAP-2 | | Cbl | | protein degradation |
| Issue Date: | 26-Jun-2009 |
| Publisher: | Academic Press |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 384 |
| Issue: | 2 |
| Start Page: | 187 |
| End Page: | 192 |
| Publisher DOI: | 10.1016/j.bbrc.2009.04.109 |
| PMID: | 19401194 |
| Abstract: | Signal transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein that contains pleckstrin and Src homology 2 (SH2)-like domains as well as a YXXQ motif in its C-terminal region. Our previous study in T cells demonstrated that STAP-2 influences FAK protein levels through recruitment of E3 ubiquitin ligase, Cbl, to FAK. In the present study, we found that Cbl directly controls the protein levels and activity of STAP-2. STAP-2 physically interacted with Cbl through its PH and SH2-like domains. Small-interfering RNA-mediated reduction of endogenous Cbl restored STAP-2 protein levels. In contrast, over-expression of Cbl induced STAP-2 degradation. Importantly, Cbl-mediated regulation of STAP-2 protein levels affected Brk/STAP-2-induced STAT3 activation. These results indicate that Cbl regulates STAP-2 protein levels and Brk/STAP-2-mediated STAT3 activation. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/38461 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 正
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