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The protein content of an adaptor protein, STAP-2 is controlled by E3 ubiquitin ligase Cbl

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Title: The protein content of an adaptor protein, STAP-2 is controlled by E3 ubiquitin ligase Cbl
Other Titles: Cbl controls STAP-2 activity through protein degradation
Authors: Sekine, Yuichi Browse this author
Yamamoto, Chikako Browse this author
Ikeda, Osamu Browse this author
Muromoto, Ryuta Browse this author
Nanbo, Asuka Browse this author
Oritani, Kenji Browse this author
Yoshimura, Akihiko Browse this author
Matsuda, Tadashi Browse this author →KAKEN DB
Keywords: STAP-2
Cbl
protein degradation
Issue Date: 26-Jun-2009
Publisher: Academic Press
Journal Title: Biochemical and Biophysical Research Communications
Volume: 384
Issue: 2
Start Page: 187
End Page: 192
Publisher DOI: 10.1016/j.bbrc.2009.04.109
PMID: 19401194
Abstract: Signal transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein that contains pleckstrin and Src homology 2 (SH2)-like domains as well as a YXXQ motif in its C-terminal region. Our previous study in T cells demonstrated that STAP-2 influences FAK protein levels through recruitment of E3 ubiquitin ligase, Cbl, to FAK. In the present study, we found that Cbl directly controls the protein levels and activity of STAP-2. STAP-2 physically interacted with Cbl through its PH and SH2-like domains. Small-interfering RNA-mediated reduction of endogenous Cbl restored STAP-2 protein levels. In contrast, over-expression of Cbl induced STAP-2 degradation. Importantly, Cbl-mediated regulation of STAP-2 protein levels affected Brk/STAP-2-induced STAT3 activation. These results indicate that Cbl regulates STAP-2 protein levels and Brk/STAP-2-mediated STAT3 activation.
Type: article (author version)
URI: http://hdl.handle.net/2115/38461
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松田 正

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