HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
水産科学院・水産科学研究院  >
雑誌発表論文等  >

Characterization of structural proteins of hirame rhabdovirus, HRV

yoshimizu-90.pdf572.28 kBPDF見る/開く

タイトル: Characterization of structural proteins of hirame rhabdovirus, HRV
著者: Nishizawa, Toyohiko 著作を一覧する
Yoshimizu, Mamoru 著作を一覧する
Winton, James 著作を一覧する
Ahne, Winfried 著作を一覧する
Kimura, Takahisa 著作を一覧する
発行日: 1991年 5月 8日
出版者: Inter-Research
誌名: Diseases of Aquatic Organisms
巻: 10
開始ページ: 167
終了ページ: 172
抄録: Structural proteins of hirame rhabdovirus (HRV) were analyzed by SDS-polyacrylamide gel electrophoresis, western blotting, 2-dimensional gel electrophoresis, and Triton X-100 treatment. Purified HRV virions were composed of: polymerase (L), glycoprotein (G), nucleoprotein (N), and 2 matrix proteins (M1 and M2). Based upon their relative mobilities, the estimated molecular weights of the proteins were: L, 156 KDa; G, 68 KDa; N, 46.4 KDa; M1, 26.4 KDa; and M2, 19.9 KDa. The electrophoretic pattern formed by the structural proteins of HRV was clearly different from that formed by pike fry rhabdovirus, spring viremia of carp virus, eel virus of America, and eel virus European X which belong to the Vesiculovirus genus; however, it resembled the pattern formed by structural proteins of viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV) which are members of the Lyssavirus genus. Among HRV, IHNV, and VHSV, differences were observed in the relative mobilities of the G, N, M1, and M2 proteins. Western blot analysis revealed that the G, N, and M2 proteins of HRV shared antigenic determinants with IHNV and VHSV, but not with any of the 4 fish vesiculoviruses tested. Cross-reactions between the M1 proteins of HRV, IHNV, or VHSV were not detected in this assay. Two-dimensional gel electrophoresis was used to show that HRV differed from IHNV or VHSV in the isoelectric point (pI) of the M1 and M2 proteins. In this system, 2 forms of the M1 protein of HRV and IHNV were observed. These subspecies of M1 had the same relative mobility but different pI values. Treatment of purified virions with 2% Triton X-100 in Tris buffer containing NaCl removed the G, M1, and M2 proteins of IHNV, but HRV virions were more stable under these conditions.
Rights: Copyright © 1991 Inter-Research
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 吉水 守


本サイトに関するご意見・お問い合わせは repo at へお願いします。 - 北海道大学