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Dual production of poly(3-hydroxybutyrate) and glutamate using variable biotin concentrations in Corynebacterium glutamicum

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Title: Dual production of poly(3-hydroxybutyrate) and glutamate using variable biotin concentrations in Corynebacterium glutamicum
Authors: Jo, Sung-Jin Browse this author
Leong, Chean Ring Browse this author
Matsumoto, Ken'ichiro Browse this author
Taguchi, Seiichi Browse this author
Keywords: Biotin
Corynebacterium glutamicum
Dual production
Glutamate
Poly(3-hydroxybutyrate)
Issue Date: Apr-2009
Publisher: Elsevier B.V.
Journal Title: Journal of Bioscience and Bioengineering
Volume: 107
Issue: 4
Start Page: 409
End Page: 411
Publisher DOI: 10.1016/j.jbiosc.2008.12.003
PMID: 19332300
Abstract: We previously synthesized poly(3-hydroxybutyrate) [P(3HB)] in recombinant Corynebacterium glutamicum, a prominent producer of amino acids. In this study, a two-step cultivation was established for the dual production of glutamate and P(3HB) due to the differences in the optimal concentration of biotin. Glutamate was extracellularly produced first under the biotin-limited condition of 0.3 μg/L. Production was then shifted to P(3HB) by addition of biotin to a total concentration of 9 μg/L. The final products obtained were 18 g/L glutamate and 36 wt% of P(3HB).
Type: article (author version)
URI: http://hdl.handle.net/2115/38592
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田口 精一

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