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Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity

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Title: Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity
Authors: Kamiya, Hiroyuki Browse this author
Fukunaga, Satoki Browse this author
Ohyama, Takashi Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: left-handedly curved DNA
histones
plasmid DNA
intranuclear disposition
carriers
Issue Date: 6-Jul-2009
Publisher: Elsevier B.V.
Journal Title: International Journal of Pharmaceutics
Volume: 376
Issue: 1-2
Start Page: 99
End Page: 103
Publisher DOI: 10.1016/j.ijpharm.2009.04.032
PMID: 19409470
Abstract: The intranuclear disposition of plasmid DNA is highly important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on transgene expression were examined in COS-7 cells with two kinds of carriers (Lipofectamine Plus and TransIT-LT1). Three plasmids containing the curved sequence at different positions were transfected. The transgene expression was affected by the position of the left-handedly curved sequence, and the sequence at appropriate locations enhanced the expression from plasmid DNAs. However, the position effects on the expression differed from those obtained by electroporation of the same plasmid DNAs in a naked form. In addition, the degree of expression enhancement seemed to depend on the carriers. These results suggest that the left-handedly curved sequence with high histone affinity could increase the transgene expression from a plasmid delivered with carriers.
Type: article (author version)
URI: http://hdl.handle.net/2115/38635
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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