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Effects of Target Sequence and Sense versus Anti-sense Strands on Gene Correction with Single-stranded DNA Fragments

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Title: Effects of Target Sequence and Sense versus Anti-sense Strands on Gene Correction with Single-stranded DNA Fragments
Authors: Kamiya, Hiroyuki Browse this author
Uchiyama, Masayuki Browse this author
Nakatsu, Yoshimichi Browse this author
Tsuzuki, Teruhisa Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: gene correction
genetic engineering
nucleic acid therapeutics
rpsL gene
single-stranded DNA fragment
Issue Date: Oct-2008
Publisher: The Japanese Biochemical Society
Journal Title: Journal of Biochemistry
Volume: 144
Issue: 4
Start Page: 431
End Page: 436
Publisher DOI: 10.1093/jb/mvn085
PMID: 18586771
Abstract: The correction of an inactivated hygromycin resistance and enhanced green fluorescent protein (Hyg–EGFP) fusion gene by a several hundred-base single-stranded (ss) DNA fragment has been reported. In this study, the effectiveness of this type of gene correction was examined for various positions in the rpsL gene. Sense and anti-sense ssDNA fragments were prepared, and the gene correction efficiencies were determined by co-introduction of the target plasmid containing the gene with the ssDNA fragments. The gene correction efficiency varied (0.8–9.3%), depending on target positions and sense/anti-sense strands. Sense ssDNA fragments corrected the target gene with equal or higher efficiencies as compared to their anti-sense counterparts. The target positions corrected with high efficiency by the sense fragments also tended to be corrected efficiently by the anti-sense fragments. These results suggest that the sense ssDNA fragments are useful for the correction of mutated genes. The variation in the correction efficiency may depend on the sequence of the target position in double-stranded DNA.
Rights: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Biochemistry following peer review. The definitive publisher-authenticated version "Journal of Biochemistry", 2008, 144(4), pp.431-436 is available online at:
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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