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Enhanced antigen presentation and CTL activity by transduction of mature rather than immature dendritic cells with octaarginine-modified liposomes

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Title: Enhanced antigen presentation and CTL activity by transduction of mature rather than immature dendritic cells with octaarginine-modified liposomes
Authors: Homhuan, Atthachai Browse this author
Kogure, Kentaro Browse this author
Nakamura, Takashi Browse this author
Shastri, Nilabh Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: Dendritic cells
Protein transduction
Octaarginine-modified liposomes
Antigen presentation
Cytotoxic T lymphocytes
Issue Date: 21-May-2009
Publisher: Elsevier B.V.
Journal Title: Journal of Controlled Release
Volume: 136
Issue: 1
Start Page: 79
End Page: 85
Publisher DOI: 10.1016/j.jconrel.2009.01.004
PMID: 19344678
Abstract: To improve uptake and cross-presentation of exogenous antigens (Ag) by dendritic cells (DCs), octaarginine-modified liposomes (R8-Lip) were used as a novel strategy for protein-Ag transduction. Immature DCs endocytose macromolecules efficiently. While mature DCs lose their ability to capture Ag, but have an increased capacity for T-cell activation. Thus Ag-transduction has been performed mostly in immature DCs. In the present study, R8-Lip were efficiently taken up by both immature and mature DCs. DCs transduced after maturation were highly efficient at cross-presentation of Ag and induced higher cytotoxic T-lymphocytes (CTL) activity than were DCs transduced before maturation. The mechanism of Ag presentation involved the escape of R8-Lip from endosomes to cytosol, which require the acidic environment. The Ag released was then processed by a proteasome-dependent pathway. This novel transduction approach is clinically applicable, easy to perform, and has more practical advantages than current protein transduction methods.
Type: article (author version)
URI: http://hdl.handle.net/2115/38744
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 原島 秀吉

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