|
Hokkaido University Collection of Scholarly and Academic Papers >
Faculty of Pharmaceutical Sciences >
Peer-reviewed Journal Articles, etc >
Enhanced antigen presentation and CTL activity by transduction of mature rather than immature dendritic cells with octaarginine-modified liposomes
| Title: | Enhanced antigen presentation and CTL activity by transduction of mature rather than immature dendritic cells with octaarginine-modified liposomes |
| Authors: | Homhuan, Atthachai Browse this author | | Kogure, Kentaro Browse this author | | Nakamura, Takashi Browse this author | | Shastri, Nilabh Browse this author | | Harashima, Hideyoshi Browse this author →KAKEN DB |
| Keywords: | Dendritic cells | | Protein transduction | | Octaarginine-modified liposomes | | Antigen presentation | | Cytotoxic T lymphocytes |
| Issue Date: | 21-May-2009 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Journal of Controlled Release |
| Volume: | 136 |
| Issue: | 1 |
| Start Page: | 79 |
| End Page: | 85 |
| Publisher DOI: | 10.1016/j.jconrel.2009.01.004 |
| PMID: | 19344678 |
| Abstract: | To improve uptake and cross-presentation of exogenous antigens (Ag) by dendritic cells (DCs), octaarginine-modified liposomes (R8-Lip) were used as a novel strategy for protein-Ag transduction. Immature DCs endocytose macromolecules efficiently. While mature DCs lose their ability to capture Ag, but have an increased capacity for T-cell activation. Thus Ag-transduction has been performed mostly in immature DCs. In the present study, R8-Lip were efficiently taken up by both immature and mature DCs. DCs transduced after maturation were highly efficient at cross-presentation of Ag and induced higher cytotoxic T-lymphocytes (CTL) activity than were DCs transduced before maturation. The mechanism of Ag presentation involved the escape of R8-Lip from endosomes to cytosol, which require the acidic environment. The Ag released was then processed by a proteasome-dependent pathway. This novel transduction approach is clinically applicable, easy to perform, and has more practical advantages than current protein transduction methods. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/38744 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 原島 秀吉
|
