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Effects of non-B DNA sequences on transgene expression

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Title: Effects of non-B DNA sequences on transgene expression
Authors: Kamiya, Hiroyuki Browse this author
Goto, Hitomi Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: non-B DNA
transgene expression
nonviral vector
Issue Date: Jul-2009
Publisher: The Society for Biotechnology, Japan
Journal Title: Journal of Bioscience and Bioengineering
Volume: 108
Issue: 1
Start Page: 20
End Page: 23
Publisher DOI: 10.1016/j.jbiosc.2009.02.013
PMID: 19577186
Abstract: DNA conformation may be an important factor affecting gene transcription. In this study, we examined how DNA sequences with unusual conformations affect transgene expression. A30 and (CG)15 sequences that can adopt the B' and Z conformations, respectively, were introduced into a β-actin promoter. Luciferase plasmids containing the manipulated promoter were transfected into NIH3T3 cells by electroporation and were delivered into mouse livers with a hydrodynamics-based injection. Expression from plasmid with the (CG)15 sequence was multiple times higher than expression from control plasmid DNA. The A30 sequence also tended to enhance expression. These results suggest that non-B DNA sequences could improve transgene expression in cells.
Rights: © 2009 The Society for Biotechnology, Japan
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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