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Hokkaido University Collection of Scholarly and Academic Papers >
Institute of Low Temperature Science >
低温科学 = Low Temperature Science >
第67巻 >
核ゲノム
| Title: | 核ゲノム |
| Other Titles: | Agrobacterium-mediated tobacco nuclear transformation |
| Authors: | 長尾, 一生1 Browse this author | | 山本, 義治2 Browse this author | | 小保方, 潤一3 Browse this author |
| Authors(alt): | Nagao, Issei1 | | Yamamoto, Yoshiharu Y.2 | | Obokata, Junichi3 |
| Issue Date: | 31-Mar-2009 |
| Publisher: | 北海道大学低温科学研究所 |
| Citation: | 光合成研究法. 北海道大学低温科学研究所, 日本光合成研究会共編 |
| Journal Title: | 低温科学 |
| Journal Title(alt): | Low Temperature Science |
| Volume: | 67 |
| Start Page: | 617 |
| End Page: | 621 |
| Abstract: | ここではアグロバクテリウムを用いたタバコの核ゲノムの形質転換について紹介する．タバコは葉緑体の形質転換も可能な数少ない植物であり，また大きく育つので生化学実験にも適している．無傷葉緑体も簡単に調製出来る． | | Tobacco is a model plant that can be used for both nuclear and chloroplast transformation. In this chapter, we introduce well established methods for Agrobacterium-mediated transformation of the tobacco nucleus. The protocol includes introduction of a binary vector into Agrobacterium, inoculation of tobacco leaf disks with Agrobacterium, and selection and regeneration of transformed tobacco. |
| Description: | 5章 形質転換 6. タバコ形質転換 a |
| Type: | bulletin (article) |
| URI: | http://hdl.handle.net/2115/39203 |
| Appears in Collections: | 低温科学 = Low Temperature Science > 第67巻
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Submitter: 低温科学研究所図書室
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