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Volume 57, Number 2 >

Generation of congenic mouse strains by introducing the virus-resistant genes, Mx1 and Oas1b, of feral mouse-derived inbred strain MSM/Ms into the common strain C57BL/6J

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.57.2.89

Title: Generation of congenic mouse strains by introducing the virus-resistant genes, Mx1 and Oas1b, of feral mouse-derived inbred strain MSM/Ms into the common strain C57BL/6J
Authors: Moritoh, Kanako Browse this author
Yamauchi, Hideto Browse this author
Asano, Atsushi Browse this author
Yoshii, Kentaro Browse this author →KAKEN DB
Kariwa, Hiroaki Browse this author
Takashima, Ikuo Browse this author
Isoda, Norikazu Browse this author
Sakoda, Yoshihiro Browse this author →KAKEN DB
Kida, Hiroshi Browse this author →KAKEN DB
Sasaki, Nobuya Browse this author
Agui, Takashi Browse this author →KAKEN DB
Keywords: congenic mouse
flavivirus
Mx1
Oas1b
orthomyxovirus
Issue Date: Aug-2009
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 57
Issue: 2
Start Page: 89
End Page: 99
Abstract: Mx1 (Myxovirus resistance protein) and Oas1b (Oligoadenylate synthetase-1), induced by type 1 interferon (IFN), play a role in early antiviral innate immunity by inhibiting the replication of viruses. In mice, Mx1 and Oas1b confer resistance to the infection of orthomyxoviruses including influenza viruses and flaviviruses including West Nile viruses, respectively. Laboratory mice have been used to study the mechanisms of the pathogenesis of these virus infections; however, it is possible that they are not a suitable model system to study these viruses, since most of the inbred laboratory mouse strains lack both genes. It has been reported that feral mouse-derived inbred strains show resistance to the infection of these viruses due to the presence of intact both genes. In this study, we generated congenic strains in which the Mx or Oas locus of the MSM/Ms (MSM) mouce was introduced to the most widely used mouse strain, C57BL/6J (B6). B6.MSM-Mx mice showed resistance to the infection of influenza virus but not of West Nile virus. On the other hand, B6.MSM-Oas mice showed resistance to the infection of West Nile virus but not of influenza virus. Our results indicate that Mx1 and Oas1b show highly antiviral specificity in mice possessing the same genetic background. Therefore, these congenic mice are useful for not only infection study but also investigation of host defense mechanism to these viruses.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/39327
Appears in Collections:Japanese Journal of Veterinary Research > Volume 57, Number 2

Submitter: 獣医学部図書室

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