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NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity
| Title: | NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity |
| Authors: | Kamiya, Hiroyuki Browse this author | | Hori, Mika Browse this author | | Arimori, Takao Browse this author | | Sekiguchi, Mutsuo Browse this author | | Yamagata, Yuriko Browse this author | | Harashima, Hideyoshi Browse this author →KAKEN DB |
| Keywords: | NUDT5 | | Oxidized nucleotide | | Nucleotide pool sanitization | | Substrate recognition |
| Issue Date: | 2-Oct-2009 |
| Publisher: | Elsevier B.V. |
| Journal Title: | DNA Repair |
| Volume: | 8 |
| Issue: | 10 |
| Start Page: | 1250 |
| End Page: | 1254 |
| Publisher DOI: | 10.1016/j.dnarep.2009.07.011 |
| PMID: | 19699693 |
| Abstract: | The human NUDT5 protein catalyzes the hydrolysis of 8-hydroxy-dGDP. To examine its substrate specificity, four oxidized deoxyribonucleotides (2-hydroxy-dADP, 8-hydroxy-dADP, 5-formyl-dUDP, and 5-hydroxy-dCDP) were incubated with the NUDT5 protein. Interestingly, all of the nucleotides, except for 5-hydroxy-dCDP, were hydrolyzed with various efficiencies. The kinetic parameters indicated that 8-hydroxy-dADP was hydrolyzed as efficiently as 8-hydroxy-dGDP. The hydrolyzing activities for their triphosphate counterparts were quite weak. These results suggest that the NUDT5 protein eliminates various oxidized deoxyribonucleoside diphosphates from the nucleotide pool and prevents their toxic effects. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/39841 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 紙谷 浩之
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