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NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity

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タイトル: NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity
著者: Kamiya, Hiroyuki 著作を一覧する
Hori, Mika 著作を一覧する
Arimori, Takao 著作を一覧する
Sekiguchi, Mutsuo 著作を一覧する
Yamagata, Yuriko 著作を一覧する
Harashima, Hideyoshi 著作を一覧する
キーワード: NUDT5
Oxidized nucleotide
Nucleotide pool sanitization
Substrate recognition
発行日: 2009年10月 2日
出版者: Elsevier B.V.
誌名: DNA Repair
巻: 8
号: 10
開始ページ: 1250
終了ページ: 1254
出版社 DOI: 10.1016/j.dnarep.2009.07.011
抄録: The human NUDT5 protein catalyzes the hydrolysis of 8-hydroxy-dGDP. To examine its substrate specificity, four oxidized deoxyribonucleotides (2-hydroxy-dADP, 8-hydroxy-dADP, 5-formyl-dUDP, and 5-hydroxy-dCDP) were incubated with the NUDT5 protein. Interestingly, all of the nucleotides, except for 5-hydroxy-dCDP, were hydrolyzed with various efficiencies. The kinetic parameters indicated that 8-hydroxy-dADP was hydrolyzed as efficiently as 8-hydroxy-dGDP. The hydrolyzing activities for their triphosphate counterparts were quite weak. These results suggest that the NUDT5 protein eliminates various oxidized deoxyribonucleoside diphosphates from the nucleotide pool and prevents their toxic effects.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 紙谷 浩之


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