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NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity

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Title: NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity
Authors: Kamiya, Hiroyuki Browse this author
Hori, Mika Browse this author
Arimori, Takao Browse this author
Sekiguchi, Mutsuo Browse this author
Yamagata, Yuriko Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: NUDT5
Oxidized nucleotide
Nucleotide pool sanitization
Substrate recognition
Issue Date: 2-Oct-2009
Publisher: Elsevier B.V.
Journal Title: DNA Repair
Volume: 8
Issue: 10
Start Page: 1250
End Page: 1254
Publisher DOI: 10.1016/j.dnarep.2009.07.011
PMID: 19699693
Abstract: The human NUDT5 protein catalyzes the hydrolysis of 8-hydroxy-dGDP. To examine its substrate specificity, four oxidized deoxyribonucleotides (2-hydroxy-dADP, 8-hydroxy-dADP, 5-formyl-dUDP, and 5-hydroxy-dCDP) were incubated with the NUDT5 protein. Interestingly, all of the nucleotides, except for 5-hydroxy-dCDP, were hydrolyzed with various efficiencies. The kinetic parameters indicated that 8-hydroxy-dADP was hydrolyzed as efficiently as 8-hydroxy-dGDP. The hydrolyzing activities for their triphosphate counterparts were quite weak. These results suggest that the NUDT5 protein eliminates various oxidized deoxyribonucleoside diphosphates from the nucleotide pool and prevents their toxic effects.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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