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Chemo-enzymatic synthesis of polyhydroxyalkanoate by an improved two-phase reaction system (TPRS)
| Title: | Chemo-enzymatic synthesis of polyhydroxyalkanoate by an improved two-phase reaction system (TPRS) |
| Authors: | Han, Xuerong Browse this author | | Satoh, Yasuharu Browse this author →KAKEN DB | | Tajima, Kenji Browse this author →KAKEN DB | | Matsushima, Tokuo Browse this author | | Munekata, Masanobu Browse this author |
| Keywords: | Chemo-enzymatic synthesis | | Polyhydroxyalkanoate (PHA) | | Two-phase reaction system (TPRS) | | PropionylCoA transferase (PCT) | | PHA synthase |
| Issue Date: | Dec-2009 |
| Publisher: | The Society for Biotechnology, Japan |
| Journal Title: | Journal of Bioscience and Bioengineering |
| Volume: | 108 |
| Issue: | 6 |
| Start Page: | 517 |
| End Page: | 523 |
| Publisher DOI: | 10.1016/j.jbiosc.2009.06.004 |
| PMID: | 19914586 |
| Abstract: | In our previous paper, we synthesized poly-3-hydroxybutyrate [P(3HB)] by using the water-organic solvent two-phase reaction system (TPRS), in which thiophenyl (R)-3-hydroxybutyrate [(R)-3HBTP] was used as a precursor of 3HBCoA. We have developed an improved TPRS for the chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA). In this method, acetyl thioester of ethyl thioglycolate (AcETG) was used as a precursor of acetylCoA (AcCoA), which was a donor of CoA. The AcCoA was formed by the ester exchange reaction between CoA in the water phase and AcETG in the organic solvent phase. The AcCoA and free 3-hydroxybutyrate (3HB) in the water phase were converted into 3HBCoA and acetate by a CoA-transfer reaction of propionylCoA transferase (PCT). The synthesized 3HBCoA was polymerized sequentially by PHA synthase, and P(3HB) was successfully formed. The maximal yield of P(3HB) was 1.2 g/l under the optimal reaction condition; this is comparable to that of in vivo PHA production. Furthermore, the number of enzymes was reduced and enzyme preparation was simplified by the construction of a fusion protein, PCT-PhaC. The chemo-enzymatic synthesis of P(3HB-co-3-hydroxypropionate) and P(3HB-co-3-mercaptopropionate) was also achieved by the improved TPRS using the fusion protein. |
| Rights: | © 2009 The Society for Biotechnology, Japan |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/42620 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田島 健次
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