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Chemo-enzymatic synthesis of polyhydroxyalkanoate by an improved two-phase reaction system (TPRS)

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Title: Chemo-enzymatic synthesis of polyhydroxyalkanoate by an improved two-phase reaction system (TPRS)
Authors: Han, Xuerong Browse this author
Satoh, Yasuharu Browse this author →KAKEN DB
Tajima, Kenji Browse this author →KAKEN DB
Matsushima, Tokuo Browse this author
Munekata, Masanobu Browse this author
Keywords: Chemo-enzymatic synthesis
Polyhydroxyalkanoate (PHA)
Two-phase reaction system (TPRS)
PropionylCoA transferase (PCT)
PHA synthase
Issue Date: Dec-2009
Publisher: The Society for Biotechnology, Japan
Journal Title: Journal of Bioscience and Bioengineering
Volume: 108
Issue: 6
Start Page: 517
End Page: 523
Publisher DOI: 10.1016/j.jbiosc.2009.06.004
PMID: 19914586
Abstract: In our previous paper, we synthesized poly-3-hydroxybutyrate [P(3HB)] by using the water-organic solvent two-phase reaction system (TPRS), in which thiophenyl (R)-3-hydroxybutyrate [(R)-3HBTP] was used as a precursor of 3HBCoA. We have developed an improved TPRS for the chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA). In this method, acetyl thioester of ethyl thioglycolate (AcETG) was used as a precursor of acetylCoA (AcCoA), which was a donor of CoA. The AcCoA was formed by the ester exchange reaction between CoA in the water phase and AcETG in the organic solvent phase. The AcCoA and free 3-hydroxybutyrate (3HB) in the water phase were converted into 3HBCoA and acetate by a CoA-transfer reaction of propionylCoA transferase (PCT). The synthesized 3HBCoA was polymerized sequentially by PHA synthase, and P(3HB) was successfully formed. The maximal yield of P(3HB) was 1.2 g/l under the optimal reaction condition; this is comparable to that of in vivo PHA production. Furthermore, the number of enzymes was reduced and enzyme preparation was simplified by the construction of a fusion protein, PCT-PhaC. The chemo-enzymatic synthesis of P(3HB-co-3-hydroxypropionate) and P(3HB-co-3-mercaptopropionate) was also achieved by the improved TPRS using the fusion protein.
Rights: © 2009 The Society for Biotechnology, Japan
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田島 健次

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