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Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition

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Title: Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition
Authors: Nakamura, Akiyoshi Browse this author
Sheppard, Kelly Browse this author
Yamane, Junji Browse this author
Yao, Min Browse this author
Söll, Dieter Browse this author
Tanaka, Isao Browse this author
Issue Date: Jan-2010
Publisher: Oxford University Press
Journal Title: Nucleic Acids Research
Volume: 38
Issue: 2
Start Page: 672
End Page: 682
Publisher DOI: 10.1093/nar/gkp955
Abstract: In many prokaryotes the biosynthesis of the amide aminoacyl-tRNAs, Gln-tRNA^[Gln] and Asn-tRNA^[Asn], proceeds by an indirect route in which mischarged Glu-tRNA^[Gln] or Asp-tRNA^[Asn] is amidated to the correct aminoacyl-tRNA catalyzed by a tRNA-dependent amidotransferase (AdT). Two types of AdTs exist: bacteria, archaea and organelles possess heterotrimeric GatCAB, while heterodimeric GatDE occurs exclusively in archaea. Bacterial GatCAB and GatDE recognize the first base pair of the acceptor stem and the D-loop of their tRNA substrates, while archaeal GatCAB recognizes the tertiary core of the tRNA, but not the first base pair. Here, we present the crystal structure of the full-length Staphylococcus aureus GatCAB. Its GatB tail domain possesses a conserved Lys rich motif that is situated close to the variable loop in a GatCAB:tRNA^[Gln] docking model. This motif is also conserved in the tail domain of archaeal GatCAB, suggesting this basic region may recognize the tRNA variable loop to discriminate Asp-tRNA^[Asn] from Asp-tRNA^[Asp] in archaea. Furthermore, we identified a 3_[10] turn in GatB that permits the bacterial GatCAB to distinguish a U1-A72 base pair from a G1-C72 pair; the absence of this element in archaeal GatCAB enables the latter enzyme to recognize aminoacyl-tRNAs with G1-C72 base pairs.
Type: article
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田中 勲

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