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Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition

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タイトル: Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition
著者: Nakamura, Akiyoshi 著作を一覧する
Sheppard, Kelly 著作を一覧する
Yamane, Junji 著作を一覧する
Yao, Min 著作を一覧する
Söll, Dieter 著作を一覧する
Tanaka, Isao 著作を一覧する
発行日: 2010年 1月
出版者: Oxford University Press
誌名: Nucleic Acids Research
巻: 38
号: 2
開始ページ: 672
終了ページ: 682
出版社 DOI: 10.1093/nar/gkp955
抄録: In many prokaryotes the biosynthesis of the amide aminoacyl-tRNAs, Gln-tRNA^[Gln] and Asn-tRNA^[Asn], proceeds by an indirect route in which mischarged Glu-tRNA^[Gln] or Asp-tRNA^[Asn] is amidated to the correct aminoacyl-tRNA catalyzed by a tRNA-dependent amidotransferase (AdT). Two types of AdTs exist: bacteria, archaea and organelles possess heterotrimeric GatCAB, while heterodimeric GatDE occurs exclusively in archaea. Bacterial GatCAB and GatDE recognize the first base pair of the acceptor stem and the D-loop of their tRNA substrates, while archaeal GatCAB recognizes the tertiary core of the tRNA, but not the first base pair. Here, we present the crystal structure of the full-length Staphylococcus aureus GatCAB. Its GatB tail domain possesses a conserved Lys rich motif that is situated close to the variable loop in a GatCAB:tRNA^[Gln] docking model. This motif is also conserved in the tail domain of archaeal GatCAB, suggesting this basic region may recognize the tRNA variable loop to discriminate Asp-tRNA^[Asn] from Asp-tRNA^[Asp] in archaea. Furthermore, we identified a 3_[10] turn in GatB that permits the bacterial GatCAB to distinguish a U1-A72 base pair from a G1-C72 pair; the absence of this element in archaeal GatCAB enables the latter enzyme to recognize aminoacyl-tRNAs with G1-C72 base pairs.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 田中 勲


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