|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition
| Title: | Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition |
| Authors: | Nakamura, Akiyoshi Browse this author | | Sheppard, Kelly Browse this author | | Yamane, Junji Browse this author | | Yao, Min Browse this author | | Söll, Dieter Browse this author | | Tanaka, Isao Browse this author |
| Issue Date: | Jan-2010 |
| Publisher: | Oxford University Press |
| Journal Title: | Nucleic Acids Research |
| Volume: | 38 |
| Issue: | 2 |
| Start Page: | 672 |
| End Page: | 682 |
| Publisher DOI: | 10.1093/nar/gkp955 |
| Abstract: | In many prokaryotes the biosynthesis of the amide aminoacyl-tRNAs, Gln-tRNA^[Gln] and Asn-tRNA^[Asn], proceeds by an indirect route in which mischarged Glu-tRNA^[Gln] or Asp-tRNA^[Asn] is amidated to the correct aminoacyl-tRNA catalyzed by a tRNA-dependent amidotransferase (AdT). Two types of AdTs exist: bacteria, archaea and organelles possess heterotrimeric GatCAB, while heterodimeric GatDE occurs exclusively in archaea. Bacterial GatCAB and GatDE recognize the first base pair of the acceptor stem and the D-loop of their tRNA substrates, while archaeal GatCAB recognizes the tertiary core of the tRNA, but not the first base pair. Here, we present the crystal structure of the full-length Staphylococcus aureus GatCAB. Its GatB tail domain possesses a conserved Lys rich motif that is situated close to the variable loop in a GatCAB:tRNA^[Gln] docking model. This motif is also conserved in the tail domain of archaeal GatCAB, suggesting this basic region may recognize the tRNA variable loop to discriminate Asp-tRNA^[Asn] from Asp-tRNA^[Asp] in archaea. Furthermore, we identified a 3_[10] turn in GatB that permits the bacterial GatCAB to distinguish a U1-A72 base pair from a G1-C72 pair; the absence of this element in archaeal GatCAB enables the latter enzyme to recognize aminoacyl-tRNAs with G1-C72 base pairs. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/42677 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 田中 勲
|
