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Apoptosis-Inducing Effect of Fucoxanthin on Human Leukemia Cell Line HL-60
Title: | Apoptosis-Inducing Effect of Fucoxanthin on Human Leukemia Cell Line HL-60 |
Authors: | Hosokawa, Masashi Browse this author →KAKEN DB | Wanezaki, Satoshi Browse this author | Miyauchi, Kozue Browse this author | Kurihara, Hideyuki Browse this author →KAKEN DB | Kohno, Hiroyuki Browse this author | Kawabata, Jun Browse this author | Odashima, Shizuo Browse this author | Takahashi, Koretaro Browse this author →KAKEN DB |
Keywords: | fucoxanthin | antiproliferation | apoptosis | DNA fragmentation | HL-60 cell |
Issue Date: | 1999 |
Publisher: | Japanese Society for Food Science and Technology |
Journal Title: | Food Science and Technology Research |
Volume: | 5 |
Issue: | 3 |
Start Page: | 243 |
End Page: | 246 |
Publisher DOI: | 10.3136/fstr.5.243 |
Abstract: | The apoptosis-inducing effect of fucoxanthin on human leukemic HL-60 cells was investigated. Fucoxanthin, which was obtained from the brown alga Undaria pinnatifida, inhibited the proliferation of HL-60 cells and induced DNA fragmentation on agarose gel electrophoresis, a typical characteristic feature of apoptotic cells. The results of sandwich ELISA using anti-biotin-antibody and anti-DNA-antibody also demonstrated DNA fragmentation in accordance with the increase in fucoxanthin concentration and incubation time. Since the level of DNA fragmentation did not increase after 24 h incubation, fucoxanthin appeared to have some effect on cell cycle. In contrast, β-carotene did not show an apoptosis-inducing effect on HL-60 cells. These results suggested that a carotenoid structure might be crucial for inducing apoptosis. |
Description: | Note |
Type: | article |
URI: | http://hdl.handle.net/2115/42695 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高橋 是太郎
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