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Contribution of cellular contractility to spatial and temporal variations in cellular stiffness
| Title: | Contribution of cellular contractility to spatial and temporal variations in cellular stiffness |
| Authors: | Nagayama, Masafumi1 Browse this author →KAKEN DB | | Haga, Hisashi2 Browse this author →KAKEN DB | | Takahashi, Masayuki3 Browse this author →KAKEN DB | | Saitoh, Takayuki4 Browse this author | | Kawabata, Kazushige5 Browse this author |
| Authors(alt): | 永山, 昌史1 | | 芳賀, 永2 | | 高橋, 正行3 | | 斉藤, 隆幸4 | | 川端, 和重5 |
| Keywords: | Fibroblast | | Scanning probe microscopy | | Atomic force microscopy | | Lysophosphatidic acid | | Y-27632 | | Stress fiber | | Actomyosin | | Mechanical stability |
| Issue Date: | 1-Nov-2004 |
| Publisher: | Elsevier Inc. |
| Journal Title: | Experimental Cell Research |
| Volume: | 300 |
| Issue: | 2 |
| Start Page: | 396 |
| End Page: | 405 |
| Publisher DOI: | 10.1016/j.yexcr.2004.07.034 |
| PMID: | 15475004 |
| Abstract: | Scanning probe microscopy and immunofluorescence observations indicated that cellular stiffness was attributed to a contractile network structure consisting of stress fibers. We measured temporal variations in cellular stiffness when cellular contractility was regulated by dosing with lysophosphatidic acid or Y-27632. This experiment reveals a clear relation between cellular stiffness and contractility: Increases in contractility cause cells to stiffen. On the other hand, decreases in contractility reduce cellular stiffness. In both cases, not only the stiffness of the stress fibers but also that of the whole of the cell varies. Immunofluorescence observations of myosin II and vinculin indicated that the stiffness variations induced by the regulation of cellular contractility were mainly due to rearrangements of the contractile actin network on the dorsal surface. Taken together, our findings provide evidence that the actin cytoskeletal network and its contractility features provide and modulate the mechanical stability of adherent cells. |
| Relation: | http://www.sciencedirect.com/science/journal/00144827 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/428 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 永山 昌史
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