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Contribution of cellular contractility to spatial and temporal variations in cellular stiffness

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Title: Contribution of cellular contractility to spatial and temporal variations in cellular stiffness
Authors: Nagayama, Masafumi1 Browse this author →KAKEN DB
Haga, Hisashi2 Browse this author →KAKEN DB
Takahashi, Masayuki3 Browse this author →KAKEN DB
Saitoh, Takayuki4 Browse this author
Kawabata, Kazushige5 Browse this author
Authors(alt): 永山, 昌史1
芳賀, 永2
高橋, 正行3
斉藤, 隆幸4
川端, 和重5
Keywords: Fibroblast
Scanning probe microscopy
Atomic force microscopy
Lysophosphatidic acid
Stress fiber
Mechanical stability
Issue Date: 1-Nov-2004
Publisher: Elsevier Inc.
Journal Title: Experimental Cell Research
Volume: 300
Issue: 2
Start Page: 396
End Page: 405
Publisher DOI: 10.1016/j.yexcr.2004.07.034
PMID: 15475004
Abstract: Scanning probe microscopy and immunofluorescence observations indicated that cellular stiffness was attributed to a contractile network structure consisting of stress fibers. We measured temporal variations in cellular stiffness when cellular contractility was regulated by dosing with lysophosphatidic acid or Y-27632. This experiment reveals a clear relation between cellular stiffness and contractility: Increases in contractility cause cells to stiffen. On the other hand, decreases in contractility reduce cellular stiffness. In both cases, not only the stiffness of the stress fibers but also that of the whole of the cell varies. Immunofluorescence observations of myosin II and vinculin indicated that the stiffness variations induced by the regulation of cellular contractility were mainly due to rearrangements of the contractile actin network on the dorsal surface. Taken together, our findings provide evidence that the actin cytoskeletal network and its contractility features provide and modulate the mechanical stability of adherent cells.
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永山 昌史

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