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DNA-Binding Property of the Novel DNA-Binding Domain STPR in FMBP-1 of the Silkworm Bombyx mori

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Title: DNA-Binding Property of the Novel DNA-Binding Domain STPR in FMBP-1 of the Silkworm Bombyx mori
Authors: Takiya, Shigeharu Browse this author →KAKEN DB
Saito, Shin Browse this author
Yokoyama, Takuya Browse this author
Matsumoto, Daisuke Browse this author
Aizawa, Tomoyasu Browse this author
Kamiya, Masakatsu Browse this author
Demura, Makoto Browse this author
Kawano, Keiichi Browse this author
Keywords: Bombyx mori
DNA bending
FMBP-1
fibroin gene
STPR domain
Issue Date: Jul-2009
Publisher: Oxford University Press
Journal Title: Journal of Biochemistry
Volume: 146
Issue: 1
Start Page: 103
End Page: 111
Publisher DOI: 10.1093/jb/mvp053
PMID: 19304790
Abstract: The STPR domain is a novel DNA-binding domain composed of repeats of 23 amino-acid-long peptide found in the fibroin-modulator-binding protein-1 (FMBP-1) of the silkworm Bombyx mori. Theoretical proteins having the STPR domain are highly conserved, particularly in vertebrates, but the functions are mostly unknown. In this study, the DNA-binding property of the STPR domain in FMBP-1 was examined. Use of reagents selecting the DNA groove and an oligonucleotide in which the dA:dT pairs of the probe were replaced with dI:dC pairs in mobility shift assay demonstrated that FMBP-1 approaches DNA from the major groove. Permutation electrophoresis using probes of the same length but containing the FMBP-1-binding site at different positions showed that FMBP-1 bends DNA through its binding. To induce the sharp bend of DNA, the STPR domain alone was insufficient and the long N-terminal extending region was necessary. Moreover, the basic region extending from the N-terminus of the STPR domain stabilized the DNA binding of the STPR domain. These results suggested that DNA-binding properties of the STPR domain are affected strongly by the structure of the flanking regions in the STPR domain-containing proteins.
Rights: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Biochemistry following peer review. The definitive publisher-authenticated version, Journal of Biochemistry, 2009, 146(1):103-111 is available online at: http://dx.doi.org/10.1093/jb/mvp053
Type: article (author version)
URI: http://hdl.handle.net/2115/42860
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 滝谷 重治

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