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DNA-Binding Property of the Novel DNA-Binding Domain STPR in FMBP-1 of the Silkworm Bombyx mori
| Title: | DNA-Binding Property of the Novel DNA-Binding Domain STPR in FMBP-1 of the Silkworm Bombyx mori |
| Authors: | Takiya, Shigeharu Browse this author →KAKEN DB | | Saito, Shin Browse this author | | Yokoyama, Takuya Browse this author | | Matsumoto, Daisuke Browse this author | | Aizawa, Tomoyasu Browse this author | | Kamiya, Masakatsu Browse this author | | Demura, Makoto Browse this author | | Kawano, Keiichi Browse this author |
| Keywords: | Bombyx mori | | DNA bending | | FMBP-1 | | fibroin gene | | STPR domain |
| Issue Date: | Jul-2009 |
| Publisher: | Oxford University Press |
| Journal Title: | Journal of Biochemistry |
| Volume: | 146 |
| Issue: | 1 |
| Start Page: | 103 |
| End Page: | 111 |
| Publisher DOI: | 10.1093/jb/mvp053 |
| PMID: | 19304790 |
| Abstract: | The STPR domain is a novel DNA-binding domain composed of repeats of 23 amino-acid-long peptide found in the fibroin-modulator-binding protein-1 (FMBP-1) of the silkworm Bombyx mori. Theoretical proteins having the STPR domain are highly conserved, particularly in vertebrates, but the functions are mostly unknown. In this study, the DNA-binding property of the STPR domain in FMBP-1 was examined. Use of reagents selecting the DNA groove and an oligonucleotide in which the dA:dT pairs of the probe were replaced with dI:dC pairs in mobility shift assay demonstrated that FMBP-1 approaches DNA from the major groove. Permutation electrophoresis using probes of the same length but containing the FMBP-1-binding site at different positions showed that FMBP-1 bends DNA through its binding. To induce the sharp bend of DNA, the STPR domain alone was insufficient and the long N-terminal extending region was necessary. Moreover, the basic region extending from the N-terminus of the STPR domain stabilized the DNA binding of the STPR domain. These results suggested that DNA-binding properties of the STPR domain are affected strongly by the structure of the flanking regions in the STPR domain-containing proteins. |
| Rights: | This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Biochemistry following peer review. The definitive publisher-authenticated version, Journal of Biochemistry, 2009, 146(1):103-111 is available online at: http://dx.doi.org/10.1093/jb/mvp053 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/42860 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 滝谷 重治
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