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DNA Microarray Analysis of Whole Blood Cells and Insulin-Sensitive Tissues Reveals the Usefulness of Blood RNA Profiling as a Source of Markers for Predicting Type 2 Diabetes

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/43118

Title: DNA Microarray Analysis of Whole Blood Cells and Insulin-Sensitive Tissues Reveals the Usefulness of Blood RNA Profiling as a Source of Markers for Predicting Type 2 Diabetes
Authors: Hayashi, Yasuhiro Browse this author
Kajimoto, Kazuaki Browse this author
Iida, Shinya Browse this author
Sato, Yuichiro Browse this author
Mizufune, Shogo Browse this author
Kaji, Noritada Browse this author
Kamiya, Hiroyuki Browse this author
Baba, Yoshinobu Browse this author
Harashima, Hideyoshi Browse this author
Keywords: whole blood cell
type 2 diabetes mellitus
gene expression
DNA microarray
Otsuka Long-Evans Tokushima Fatty rat
diagnosis
Issue Date: Jun-2010
Publisher: Pharmaceutical Society of Japan
Journal Title: Biological & Pharmaceutical Bulletin
Volume: 33
Issue: 6
Start Page: 1033
End Page: 1042
Publisher DOI: 10.1248/bpb.33.1033
Abstract: To determine if gene expression profiling of whole blood cells is a useful source of markers for the early diagnosis of the onset of type 2 diabetes, we examined gene expression profiling of whole blood cells and type 2 diabetes-related organs, such as liver, adipose tissue, and skeletal muscle, of Otsuka Long-Evans Tokushima Fatty (OLETF) rats. At the age of 6 weeks, RNA was isolated from tissues of fasted OLETF and control Long-Evans Tokushima Otsuka (LETO) rats. Gene expression was analyzed using the Agilent rat oligo microarray. Gene ontology analysis showed that gene expression of biologically meaningful groups of genes in liver, adipose tissue, and skeletal muscle, which are involved in the pathogenesis of type 2 diabetes, differed between OLETF and LETO rats. Three hundred genes of whole blood cells were differentially expressed. Four out of these 300 genes were related to the insulin-signaling pathway and 57 out of 300 genes were up- or down-regulated in at least one tissues in OLETF rats. These results support our hypothesis that gene expression profiling of whole blood cells might be a useful source of markers to predict the onset of type 2 diabetes.
Type: article
URI: http://hdl.handle.net/2115/43118
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 原島 秀吉

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