Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3


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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/43144

Title:	Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3
Authors:	Kamiya, Hiroyuki Browse this author
	Goto, Hitomi Browse this author
	Kanda, Genki Browse this author
	Yamada, Yuma Browse this author
	Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords:	Exogenous DNA
	Histone
	H3
	Intranuclear disposition
	Expression efficiency
Issue Date:	15-Jun-2010
Publisher:	Elsevier B.V.
Journal Title:	International Journal of Pharmaceutics
Volume:	392
Issue:	1-2
Start Page:	249
End Page:	253
Publisher DOI:	10.1016/j.ijpharm.2010.03.035
PMID:	20362041
Abstract:	The intranuclear disposition of plasmid DNA is extremely important for transgene expression. Exogenous histones have been used as carriers of plasmid DNA in histone-mediated gene delivery. In this study, the effects of exogenous histone H3 complexed with plasmid DNA on transgene expression efficiency were examined. The plasmid-histone complexes in various ratios were transfected into HeLa cells by osmotic pressure. Histone H3 suppressed transgene expression in the nucleus in a dose-dependent manner. Our results suggest that the histone-mediated gene delivery is unlikely to be useful, from the viewpoint of the intranuclear disposition.
Type:	article (author version)
URI:	http://hdl.handle.net/2115/43144
Appears in Collections:	薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷浩之

OAI-PMH ( junii2 , jpcoar_1.0 )

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