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Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3

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Title: Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3
Authors: Kamiya, Hiroyuki Browse this author
Goto, Hitomi Browse this author
Kanda, Genki Browse this author
Yamada, Yuma Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: Exogenous DNA
Intranuclear disposition
Expression efficiency
Issue Date: 15-Jun-2010
Publisher: Elsevier B.V.
Journal Title: International Journal of Pharmaceutics
Volume: 392
Issue: 1-2
Start Page: 249
End Page: 253
Publisher DOI: 10.1016/j.ijpharm.2010.03.035
PMID: 20362041
Abstract: The intranuclear disposition of plasmid DNA is extremely important for transgene expression. Exogenous histones have been used as carriers of plasmid DNA in histone-mediated gene delivery. In this study, the effects of exogenous histone H3 complexed with plasmid DNA on transgene expression efficiency were examined. The plasmid-histone complexes in various ratios were transfected into HeLa cells by osmotic pressure. Histone H3 suppressed transgene expression in the nucleus in a dose-dependent manner. Our results suggest that the histone-mediated gene delivery is unlikely to be useful, from the viewpoint of the intranuclear disposition.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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