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An oligochaete homologue of the Brachyury gene is expressed transiently in the third quartette of micromeres
| Title: | An oligochaete homologue of the Brachyury gene is expressed transiently in the third quartette of micromeres |
| Authors: | Kitakoshi, Tomohito Browse this author | | Shimizu, Takashi Browse this author |
| Keywords: | Brachyury | | Annelid | | Tubifex tubilex | | Micromeres | | Teloblasts | | Blastopore | | Direct development |
| Issue Date: | Sep-2010 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Gene Expression Patterns |
| Volume: | 10 |
| Issue: | 6 |
| Start Page: | 306 |
| End Page: | 313 |
| Publisher DOI: | 10.1016/j.gep.2010.07.001 |
| PMID: | 20603182 |
| Abstract: | We have isolated a Brachyury homologue (Ttu-Bra) from the oligochaete annelid Tubifex tubifex which displays a direct mode of development. Developmental RT-PCR analysis showed that Ttu-Bra transcripts are present in embryos at stages 9-11 and 16-17, but undetectable at the remaining embryonic stages. Whole-mount in situ hybridization demonstrated that Ttu-Bra is expressed transiently in the third quartette of micromeres, which are located at the prospective stomodaeum (at stages 9-11). The second burst of Ttu-Bra expression occurs at the posterior end of stage 16 embryo that undergoes body elongation. Ttu-Bra-expressing cells, which are organized in a circle at stage 16, become aggregated at the proctodaeum at stage 17. Consistent with the results of the RT-PCR analysis, there is no sign of Ttu-Bra-expressing cells in embryos that undergo gastrulation during stages 12 to 15. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43800 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 清水 隆
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