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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
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Vascular Cell-Like Potential of Undifferentiated Ligament Fibroblasts to Construct Vascular Cell-Specific Marker-Positive Blood Vessel Structures in a PI3K Activation-Dependent Manner
| Title: | Vascular Cell-Like Potential of Undifferentiated Ligament Fibroblasts to Construct Vascular Cell-Specific Marker-Positive Blood Vessel Structures in a PI3K Activation-Dependent Manner |
| Authors: | Okubo, Naoto Browse this author | | Ishisaki, Akira Browse this author | | Iizuka, Tadashi Browse this author | | Tamura, Masato Browse this author | | Kitagawa, Yoshimasa Browse this author |
| Keywords: | Three-dimensional vessel reconstruction | | Angiogenesis | | Tie-2 | | FLK-1 | | Differentiation | | Fibroblasts | | Endothelial cell | | Fibroblast growth factor | | PI3K | | Periodontal ligament |
| Issue Date: | Aug-2010 |
| Publisher: | Karger |
| Journal Title: | Journal of Vascular Research |
| Volume: | 47 |
| Issue: | 5 |
| Start Page: | 369 |
| End Page: | 383 |
| Publisher DOI: | 10.1159/000277724 |
| PMID: | 20110728 |
| Abstract: | Objective: To evaluate whether fibroblasts derived from periodontal ligament (PDL) retain the ability to differentiate into putative vascular cells and construct vascular cell-specific marker-positive blood vessel structures. We also evaluated the morphological features of the structure and investigated the intracellular molecular mechanism underlying the angiogenic activity of these cells. Methods: Single cell-derived cultures (SCDCs) were established from primary rat ligament fibroblast cultures, and their expression of ligament cell-, mesenchymal stem cell (MSC)-, and vascular cell-specific markers was evaluated by RT-PCR and immunocytochemistry. The ability of the cells to construct a blood vessel structure was evaluated in a three-dimensional type I collagen scaffold. The morphological and immunohistological characteristics of the structure were then evaluated. Results: Each SCDC expressed endothelial cell (EC) and smooth muscle cell-specific markers, in addition to MSC- and ligament cell-specific markers. SCDC2 cells, which abundantly expressed the EC markers Flk-1 and Tie-2, vigorously constructed a blood vessel structure in a phosphoinositide 3-kinase activation-dependent manner. Conclusion: PDL fibroblasts have the potential to construct an EC marker-positive blood vessel-like structure. Consequently, the fibroblastic lineage in ligament tissue could be a candidate precursor for construction of a vascular system around damaged ligament tissue to facilitate its regeneration. |
| Rights: | Copyright © 2010 S. Karger AG, Basel |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/43855 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 北川 善政
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