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Vascular Cell-Like Potential of Undifferentiated Ligament Fibroblasts to Construct Vascular Cell-Specific Marker-Positive Blood Vessel Structures in a PI3K Activation-Dependent Manner

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Title: Vascular Cell-Like Potential of Undifferentiated Ligament Fibroblasts to Construct Vascular Cell-Specific Marker-Positive Blood Vessel Structures in a PI3K Activation-Dependent Manner
Authors: Okubo, Naoto Browse this author
Ishisaki, Akira Browse this author
Iizuka, Tadashi Browse this author
Tamura, Masato Browse this author
Kitagawa, Yoshimasa Browse this author
Keywords: Three-dimensional vessel reconstruction
Angiogenesis
Tie-2
FLK-1
Differentiation
Fibroblasts
Endothelial cell
Fibroblast growth factor
PI3K
Periodontal ligament
Issue Date: Aug-2010
Publisher: Karger
Journal Title: Journal of Vascular Research
Volume: 47
Issue: 5
Start Page: 369
End Page: 383
Publisher DOI: 10.1159/000277724
PMID: 20110728
Abstract: Objective: To evaluate whether fibroblasts derived from periodontal ligament (PDL) retain the ability to differentiate into putative vascular cells and construct vascular cell-specific marker-positive blood vessel structures. We also evaluated the morphological features of the structure and investigated the intracellular molecular mechanism underlying the angiogenic activity of these cells. Methods: Single cell-derived cultures (SCDCs) were established from primary rat ligament fibroblast cultures, and their expression of ligament cell-, mesenchymal stem cell (MSC)-, and vascular cell-specific markers was evaluated by RT-PCR and immunocytochemistry. The ability of the cells to construct a blood vessel structure was evaluated in a three-dimensional type I collagen scaffold. The morphological and immunohistological characteristics of the structure were then evaluated. Results: Each SCDC expressed endothelial cell (EC) and smooth muscle cell-specific markers, in addition to MSC- and ligament cell-specific markers. SCDC2 cells, which abundantly expressed the EC markers Flk-1 and Tie-2, vigorously constructed a blood vessel structure in a phosphoinositide 3-kinase activation-dependent manner. Conclusion: PDL fibroblasts have the potential to construct an EC marker-positive blood vessel-like structure. Consequently, the fibroblastic lineage in ligament tissue could be a candidate precursor for construction of a vascular system around damaged ligament tissue to facilitate its regeneration.
Rights: Copyright © 2010 S. Karger AG, Basel
Type: article (author version)
URI: http://hdl.handle.net/2115/43855
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 北川 善政

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