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Epigenetic patterns at the mouse prolyl oligopeptidase gene locus suggest the CpG island in the gene body to be a novel regulator for gene expression

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Title: Epigenetic patterns at the mouse prolyl oligopeptidase gene locus suggest the CpG island in the gene body to be a novel regulator for gene expression
Authors: Matsubara, Shin Browse this author
Takahashi, Takayuki Browse this author →KAKEN DB
Kimura, Atsushi P. Browse this author →KAKEN DB
Keywords: Prolyl oligopeptidase
Granulosa cell
Blastocyst
DNA methylation
Histone acetylation
Histone H3K4 methylation
Issue Date: 1-Oct-2010
Publisher: ELSEVIER SCIENCE BV
Journal Title: GENE
Volume: 465
Issue: 1-2
Start Page: 17
End Page: 29
Publisher DOI: 10.1016/j.gene.2010.06.006
PMID: 20600704
Abstract: Prolyl oligopeptidase (POP) is a widely distributed serine peptidase which hydrolyzes small peptides on the carboxyl side of an internal proline residue. While its physiological role has been intensely studied, the regulatory mechanism of the gene expression is poorly understood. This time we assessed the POP mRNA expression in mouse embryos and tissues related to reproduction and development and found that POP mRNA was highly expressed in the ovarian granulosa cell, placental spongiotrophoblast, and blastocyst embryo. To elucidate the mechanism by which POP expression is regulated, we investigated DNA methylation and histone modification patterns of the two CpG islands (CGIs) found at the, mouse POP locus. Whereas the CGI including the POP promoter (CGI-1) was completely hypomethylated in all the tissues examined, DNA methylation level of the CGI in the gene body (CGI-2) was lower in the granulosa cell, placenta, and blastocyst than in the liver. Some specific CpGs in CGI-2 were significantly demethylated in the three tissues. An in vitro reporter analysis indicated that CGI-2 enhanced POP promoter activity and its effect was significantly reduced by DNA methylation. Moreover, histone H3 acetylation and H3K4 methylation levels of CGI-2 were higher in the granulosa cell than liver. The results suggest that the CGI-2 region is a cis-element for the POP gene expression. (C) 2010 Elsevier B.V. All rights reserved.
Type: article (author version)
URI: http://hdl.handle.net/2115/43940
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 敦

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