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Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in Lonicera caerulea var. emphyllocalyx

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/44168

Title: Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in Lonicera caerulea var. emphyllocalyx
Authors: Miyashita, Tomomi Browse this author
Ohashi, Takafumi Browse this author
Shibata, Fukashi Browse this author
Araki, Hajime Browse this author →KAKEN DB
Hoshino, Yoichiro Browse this author
Keywords: Aneuploid
Endosperm culture
Hexaploid
Lonicera caerulea
Ploidy level
Regeneration
Issue Date: Sep-2009
Publisher: Springer Netherlands
Journal Title: Plant Cell, Tissue and Organ Culture
Volume: 98
Issue: 3
Start Page: 291
End Page: 301
Publisher DOI: 10.1007/s11240-009-9562-6
Abstract: An endosperm culture of Haskap (Lonicera caerulea var. emphyllocalyx) was established to develop polyploid plants and investigate the regeneration ability of the endosperm. Based on histological analysis of embryo and endosperm development, endosperms at the globular to early torpedo-stages of developing embryos were used to initiate an endosperm culture. Formation of shoot primordia was observed on Murashige and Skoog (MS) medium (1962) containing benzyladenine (BA) and indole-3-butyric acid (IBA). Shoot primordium formation was confirmed in some genotypes with regeneration frequencies ranging between 1.9% to 10.0%. These proliferated on 1/2 MS medium containing 2.89 μM gibberellic acid (GA3), and then elongated and rooted on MS medium containing 0.44 μM BA and 2.89 μM GA3. These shoots developed into plantlets on 1/2 MS medium. Plantlets maintained ploidy of the endosperm following flow cytometric analysis, thus confirming that these were derived from the endosperm. These results indicated that endosperms were capable of regeneration.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/44168
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 星野 洋一郎

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