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Transgenic zebrafish reveals novel mechanisms of translational control of cyclin B1 mRNA in oocytes

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Title: Transgenic zebrafish reveals novel mechanisms of translational control of cyclin B1 mRNA in oocytes
Authors: Yasuda, Kyota Browse this author
Kotani, Tomoya Browse this author
Ota, Ryoma Browse this author
Yamashita, Masakane Browse this author
Keywords: Translational control
Oocyte maturation
Cyclin B1
Transgenic zebrafish
Real-time imaging
Issue Date: 1-Dec-2010
Publisher: Elsevier
Journal Title: Developmental Biology
Volume: 348
Issue: 1
Start Page: 76
End Page: 86
Publisher DOI: 10.1016/j.ydbio.2010.09.015
PMID: 20883683
Abstract: Temporal translation control of localized mRNA is crucial for regulating various cellular and developmental processes. However, little is known about the mechanisms of temporal translation control of localized mRNA due to the limitation in technology. cyclin B1 mRNA at the animal polar cytoplasm of immature zebrafish oocytes is translationally repressed, and its activation is temporally regulated during maturation. Mechanisms of cyclin B1 translation in oocytes were analyzed using transgenic zebrafish in which reporter mRNAs are produced from transgenes introduced into the genome through transcription in the nucleus followed by transport to the cytoplasm, as in endogenous mRNAs. Real-time imaging of the site and timing of translation showed that mRNAs containing the full-length cyclin B1 sequence precisely mimic the localization and translation of endogenous cyclin B1 mRNA. However, mRNAs containing cyclin B1 3' untranslated region but lacking open reading frame (ORF) underwent abnormal localization and precocious translational activation, indicating the significance of the ORF in translational control of cyclin B1 mRNA. Our genetic approach in combination with real-time imaging of the translation site and timing provides a novel insight into the mechanisms of temporal control of translation.
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山下 正兼

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