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Spontaneous Redifferentiation of Dedifferentiated Human Articular Chondrocytes on Hydrogel Surfaces

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Title: Spontaneous Redifferentiation of Dedifferentiated Human Articular Chondrocytes on Hydrogel Surfaces
Authors: Yang, Jing Jing Browse this author
Chen, Yong Mei Browse this author
Liu, Jian Fang Browse this author
Kurokawa, Takayuki Browse this author →KAKEN DB
Gong, Jian Ping Browse this author →KAKEN DB
Issue Date: 5-Aug-2010
Publisher: Mary Ann Liebert
Journal Title: Tissue Engineering Part A
Volume: 16
Issue: 8
Start Page: 2529
End Page: 2540
Publisher DOI: 10.1089/ten.tea.2009.0647
PMID: 20233009
Abstract: Chondrocytes rapidly dedifferentiate into a more fibroblastic phenotype on a two-dimensional polystyrene substratum. This impedes fundamental research on these cells as well as their clinical application. This study investigated the redifferentiation behavior of dedifferentiated chondrocytes on a hydrogel substratum. Dedifferentiated normal human articular chondrocyte–knee (NHAC-kn) cells were released from the sixth-passage monolayer cultured on a polystyrene surface. These cells were then subcultured on a chemically crosslinked copolymer hydrogel, that is, poly(NaAMPS-co-DMAAm), and the cells thus obtained were used as the seventhpassage cultivation. Copolymer gels were synthesized from a negatively charged monomer, the sodium salt of 2-acrylamido-2-methyl-1-propanesulfonic acid (NaAMPS), and a neutral monomer, N,N-dimethylacrylamide (DMAAm). These gels were of different compositions because the molar fraction (F) of NaAMPS was varied (F¼0, 0.2, 0.4, 0.6, 0.8, and 1.0). The dedifferentiated NHAC-kn cells spontaneously redifferentiated to normal NHAC-kn cells on neutral (F¼0) and poly(NaAMPS-co-DMAAm) hydrogels of low charge density (F¼0.2). This was deduced from the cell morphology and expression of cartilage-specific genes and proteins. These results should enable us to establish a simple and efficient method for preparing large amounts of chondrocytes by cultivation on the surfaces of neutral and low-charge-density hydrogels.
Rights: This is a copy of an article published in the TISSUE ENGINEERING: Part A © 2010 Mary Ann Liebert, Inc.; TISSUE ENGINEERING: Part A is available online at:
Type: article
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 龔 剣萍 (Gong Jian Ping)

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