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Spontaneous Redifferentiation of Dedifferentiated Human Articular Chondrocytes on Hydrogel Surfaces
Title: | Spontaneous Redifferentiation of Dedifferentiated Human Articular Chondrocytes on Hydrogel Surfaces |
Authors: | Yang, Jing Jing Browse this author | Chen, Yong Mei Browse this author | Liu, Jian Fang Browse this author | Kurokawa, Takayuki Browse this author →KAKEN DB | Gong, Jian Ping Browse this author →KAKEN DB |
Issue Date: | 5-Aug-2010 |
Publisher: | Mary Ann Liebert |
Journal Title: | Tissue Engineering Part A |
Volume: | 16 |
Issue: | 8 |
Start Page: | 2529 |
End Page: | 2540 |
Publisher DOI: | 10.1089/ten.tea.2009.0647 |
PMID: | 20233009 |
Abstract: | Chondrocytes rapidly dedifferentiate into a more fibroblastic phenotype on a two-dimensional polystyrene substratum. This impedes fundamental research on these cells as well as their clinical application. This study investigated the redifferentiation behavior of dedifferentiated chondrocytes on a hydrogel substratum. Dedifferentiated normal human articular chondrocyte–knee (NHAC-kn) cells were released from the sixth-passage monolayer cultured on a polystyrene surface. These cells were then subcultured on a chemically crosslinked copolymer hydrogel, that is, poly(NaAMPS-co-DMAAm), and the cells thus obtained were used as the seventhpassage cultivation. Copolymer gels were synthesized from a negatively charged monomer, the sodium salt of 2-acrylamido-2-methyl-1-propanesulfonic acid (NaAMPS), and a neutral monomer, N,N-dimethylacrylamide (DMAAm). These gels were of different compositions because the molar fraction (F) of NaAMPS was varied (F¼0, 0.2, 0.4, 0.6, 0.8, and 1.0). The dedifferentiated NHAC-kn cells spontaneously redifferentiated to normal NHAC-kn cells on neutral (F¼0) and poly(NaAMPS-co-DMAAm) hydrogels of low charge density (F¼0.2). This was deduced from the cell morphology and expression of cartilage-specific genes and proteins. These results should enable us to establish a simple and efficient method for preparing large amounts of chondrocytes by cultivation on the surfaces of neutral and low-charge-density hydrogels. |
Rights: | This is a copy of an article published in the TISSUE ENGINEERING: Part A © 2010 Mary Ann Liebert, Inc.; TISSUE ENGINEERING: Part A is available online at: http://www.liebertonline.com. |
Type: | article |
URI: | http://hdl.handle.net/2115/44510 |
Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 龔 剣萍 (Gong Jian Ping)
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