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Study on the Sliding Friction of Endothelial Cells Cultured on Hydrogel and the Role of Glycocalyx on Friction Reduction
| Title: | Study on the Sliding Friction of Endothelial Cells Cultured on Hydrogel and the Role of Glycocalyx on Friction Reduction |
| Authors: | Chen, Yong Mei Browse this author | | Kurokawa, Takayuki Browse this author →KAKEN DB | | Tominaga, Taiki Browse this author | | Yasuda, Kazunori Browse this author →KAKEN DB | | Osada, Yoshihito Browse this author →KAKEN DB | | Gong, Jian Ping Browse this author →KAKEN DB | | Yamamoto, Kimiko Browse this author | | Ando, Joji Browse this author |
| Keywords: | Friction | | Endothelial cell | | Glycocalyx | | Gel scaffold | | Lubrication |
| Issue Date: | 17-Nov-2010 |
| Publisher: | WILEY |
| Journal Title: | Advanced Engineering Materials |
| Volume: | 12 |
| Issue: | 11 |
| Start Page: | B628 |
| End Page: | B636 |
| Publisher DOI: | 10.1002/adem.201080021 |
| Abstract: | In this study, we investigated the sliding friction of human umbilical vein endothelial cell (HUVEC) monolayer cultured on poly(sodium p-styrene sulfonate) (PNaSS) gel, intending to elucidate the role of the glycocalyx on the surface of endothelial cell (EC) in friction reduction. Three sets of HUVEC monolayers were investigated: 1) as-cultured HUVEC monolayer, 2) HUVEC monolayer treated by transforming growth factor υ1 (TGF-υ1), which increased glycocalyx by 148%, 3) HUVEC monolayer treated by heparinase I, which reduced glycocalyx by 57%, both were compared with that of the as prepared one. When being slid on flat glass surface, the frictional stress of HUVEC monolayer decreased in the order of heparinase I-treated > as-cultured > TGF-υ1-treated samples. The results suggested that glycocalyx may play a role in reducing the friction of endothelial cell monolayer. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44824 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 龔 剣萍 (Gong Jian Ping)
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