|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Gene expression, glycocalyx assay, and surface properties of human endothelial cells cultured on hydrogel matrix with sulfonic moiety : Effect of elasticity of hydrogel
| Title: | Gene expression, glycocalyx assay, and surface properties of human endothelial cells cultured on hydrogel matrix with sulfonic moiety : Effect of elasticity of hydrogel |
| Authors: | Yang, Jing Jing Browse this author | | Chen, Yong Mei Browse this author | | Kurokawa, Takayuki Browse this author →KAKEN DB | | Gong, Jian Ping Browse this author →KAKEN DB | | Onodera, Shin Browse this author →KAKEN DB | | Yasuda, Kazunori Browse this author →KAKEN DB |
| Keywords: | endothelial cells | | glycocalyx | | hydrogels | | elasticity | | platelet adhesion |
| Issue Date: | Aug-2010 |
| Publisher: | WILEY |
| Journal Title: | Journal of Biomedical Materials Research Part A |
| Volume: | 95A |
| Issue: | 2 |
| Start Page: | 531 |
| End Page: | 542 |
| Publisher DOI: | 10.1002/jbm.a.32875 |
| PMID: | 20681030 |
| Abstract: | We measured the gene expression, glycocalyx content, and surface properties of human coronary artery endothelial cells (HCAECs) cultured on poly(sodium p-styrene sulfonate) (PNaSS) hydrogels with various levels of elasticity ranged in 3 kPa ~ 300 kPa. We found that all HCAECs reached confluence on these hydrogels while retaining the similar expression of EC-specific markers to that on polystyrene (PS), a widely used scaffold in cell culture in vitro. Real-time polymerase chain reaction (PCR) and glycosaminoglycan (GAG) assay showed that the amount of EC-specific glycocalyx secreted by HCAECs cultured on PNaSS gels was higher than that cultured on PS, and it increased with an increase of gel elasticity. Furthermore, the HCAECs cultured on PNaSS gels showed excellent property against platelet adhesion and lower surface friction than that on PS. The platelet adhesion and surface friction of HCAECs cultured on PNaSS gels also depend on the elasticity of gels. The largest amount of EC-specific glycocalyx, excellent blood compatibility, and the lowest friction were observed when the elastic modulus of the gel was larger than 60 kPa. Overall, HCAECs cultured on these hydrogels have better properties than those cultured on PS scaffolds, demonstrating the PNaSS gels can be used as potential tissue engineering material for blood vessels. |
| Rights: | This is the pre-peer-reviewed version of the following article: FULL CITE, which has been published in final form at [Journal of Biomedical Materials Research Part A：Aug-2010, 95A(2), pp.531-542.] |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44840 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 龔 剣萍 (Gong Jian Ping)
|
