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Gene expression, glycocalyx assay, and surface properties of human endothelial cells cultured on hydrogel matrix with sulfonic moiety : Effect of elasticity of hydrogel

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Title: Gene expression, glycocalyx assay, and surface properties of human endothelial cells cultured on hydrogel matrix with sulfonic moiety : Effect of elasticity of hydrogel
Authors: Yang, Jing Jing Browse this author
Chen, Yong Mei Browse this author
Kurokawa, Takayuki Browse this author →KAKEN DB
Gong, Jian Ping Browse this author →KAKEN DB
Onodera, Shin Browse this author →KAKEN DB
Yasuda, Kazunori Browse this author →KAKEN DB
Keywords: endothelial cells
glycocalyx
hydrogels
elasticity
platelet adhesion
Issue Date: Aug-2010
Publisher: WILEY
Journal Title: Journal of Biomedical Materials Research Part A
Volume: 95A
Issue: 2
Start Page: 531
End Page: 542
Publisher DOI: 10.1002/jbm.a.32875
PMID: 20681030
Abstract: We measured the gene expression, glycocalyx content, and surface properties of human coronary artery endothelial cells (HCAECs) cultured on poly(sodium p-styrene sulfonate) (PNaSS) hydrogels with various levels of elasticity ranged in 3 kPa ~ 300 kPa. We found that all HCAECs reached confluence on these hydrogels while retaining the similar expression of EC-specific markers to that on polystyrene (PS), a widely used scaffold in cell culture in vitro. Real-time polymerase chain reaction (PCR) and glycosaminoglycan (GAG) assay showed that the amount of EC-specific glycocalyx secreted by HCAECs cultured on PNaSS gels was higher than that cultured on PS, and it increased with an increase of gel elasticity. Furthermore, the HCAECs cultured on PNaSS gels showed excellent property against platelet adhesion and lower surface friction than that on PS. The platelet adhesion and surface friction of HCAECs cultured on PNaSS gels also depend on the elasticity of gels. The largest amount of EC-specific glycocalyx, excellent blood compatibility, and the lowest friction were observed when the elastic modulus of the gel was larger than 60 kPa. Overall, HCAECs cultured on these hydrogels have better properties than those cultured on PS scaffolds, demonstrating the PNaSS gels can be used as potential tissue engineering material for blood vessels.
Rights: This is the pre-peer-reviewed version of the following article: FULL CITE, which has been published in final form at [Journal of Biomedical Materials Research Part A:Aug-2010, 95A(2), pp.531-542.]
Type: article (author version)
URI: http://hdl.handle.net/2115/44840
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 龔 剣萍 (Gong Jian Ping)

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