Hokkaido University Collection of Scholarly and Academic Papers >
Faculty of Pharmaceutical Sciences >
Peer-reviewed Journal Articles, etc >
AMP-activated protein kinase regulates the expression of monocarboxylate transporter 4 in skeletal muscle
Title: | AMP-activated protein kinase regulates the expression of monocarboxylate transporter 4 in skeletal muscle |
Authors: | Furugen, Ayako Browse this author | Kobayashi, Masaki Browse this author | Narumi, Katsuya Browse this author | Watanabe, Meguho Browse this author | Otake, Sho Browse this author | Itagaki, Shirou Browse this author | Iseki, Ken Browse this author |
Keywords: | AMPK | MCT4 | Up-regulation | L-lactic acid | Skeletal muscle |
Issue Date: | 17-Jan-2011 |
Publisher: | Elsevier |
Journal Title: | Life Sciences |
Volume: | 88 |
Issue: | 3-4 |
Start Page: | 163 |
End Page: | 168 |
Publisher DOI: | 10.1016/j.lfs.2010.11.003 |
PMID: | 21070787 |
Abstract: | Aims: The aim of this study was to determine the effect of 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR), an AMP-activated protein kinase (AMPK) activator, on monocarboxylate transporter 4 (MCT4) expression in rat skeletal muscle and a prototypic embryonal rhabdomyosarcoma cell line (RD cells). Main methods: We examined the alteration in Glucose transporter 4 (GLUT4) and MCT4 mRNA levels by quantitative real-time PCR. Alteration in GLUT4 and MCT4 protein levels was examined by Western blotting. Key findings: In an in vivo study, AICAR increased MCT4 mRNA and protein levels in a fiber-type specific manner. In an in vitro study, AICAR increased MCT4 mRNA and protein levels. Moreover, AICAR-induced MCT4 expression was blocked by Compound C, an AMPK inhibitor. Significance: In this study, we found that AMPK activation induced expression of MCT4 in RD cells and rat skeletal muscle in a fiber-type specific manner. These results indicate the possible involvement of an AMPK-mediated pathway associated with MCT4 expression in skeletal muscle. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/44904 |
Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 小林 正紀
|