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AMP-activated protein kinase regulates the expression of monocarboxylate transporter 4 in skeletal muscle

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Title: AMP-activated protein kinase regulates the expression of monocarboxylate transporter 4 in skeletal muscle
Authors: Furugen, Ayako Browse this author
Kobayashi, Masaki Browse this author
Narumi, Katsuya Browse this author
Watanabe, Meguho Browse this author
Otake, Sho Browse this author
Itagaki, Shirou Browse this author
Iseki, Ken Browse this author
Keywords: AMPK
L-lactic acid
Skeletal muscle
Issue Date: 17-Jan-2011
Publisher: Elsevier
Journal Title: Life Sciences
Volume: 88
Issue: 3-4
Start Page: 163
End Page: 168
Publisher DOI: 10.1016/j.lfs.2010.11.003
PMID: 21070787
Abstract: Aims: The aim of this study was to determine the effect of 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR), an AMP-activated protein kinase (AMPK) activator, on monocarboxylate transporter 4 (MCT4) expression in rat skeletal muscle and a prototypic embryonal rhabdomyosarcoma cell line (RD cells). Main methods: We examined the alteration in Glucose transporter 4 (GLUT4) and MCT4 mRNA levels by quantitative real-time PCR. Alteration in GLUT4 and MCT4 protein levels was examined by Western blotting. Key findings: In an in vivo study, AICAR increased MCT4 mRNA and protein levels in a fiber-type specific manner. In an in vitro study, AICAR increased MCT4 mRNA and protein levels. Moreover, AICAR-induced MCT4 expression was blocked by Compound C, an AMPK inhibitor. Significance: In this study, we found that AMPK activation induced expression of MCT4 in RD cells and rat skeletal muscle in a fiber-type specific manner. These results indicate the possible involvement of an AMPK-mediated pathway associated with MCT4 expression in skeletal muscle.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小林 正紀

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