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Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake
| Title: | Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake |
| Authors: | Furukawa, Ryo Browse this author | | Yamada, Yuma Browse this author | | Takenaga, Mitsuko Browse this author | | Igarashi, Rie Browse this author | | Harashima, Hideyoshi Browse this author |
| Keywords: | Lecithinized superoxide dismutase (PC-SOD) | | Octaarginine-modified liposomes | | Anti-oxidant therapy | | Reactive oxygen species (ROS) | | Mitochondria |
| Issue Date: | 21-Jan-2011 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 404 |
| Issue: | 3 |
| Start Page: | 796 |
| End Page: | 801 |
| Publisher DOI: | 10.1016/j.bbrc.2010.12.062 |
| PMID: | 21168389 |
| Abstract: | The anti-oxidant enzyme superoxide dismutase (SOD) has the potential for use as a therapeutic agent in the treatment of various diseases caused by reactive oxygen species. However, achieving this would be difficult without a suitable delivery system for SOD. We previously reported that PC-SOD, in which four molecules of a phosphatidylcholine (PC) derivative were covalently bound to each dimer of recombinant human CuZnSOD, was a high affinity for the cell membrane (R. Igarashi et al, J. Pharmacol. Exp. Ther. 271 (1994) 1672-7). Here, we show that an octaarginine (R8) modified liposome equipped with PC-SOD (R8-LP (PC-SOD)) enhances its anti-oxidant effect. High-density R8-modified liposomes can stimulate macropinocytosis and are taken up efficiently by cells as demonstrated in a previous study (I.A. Khalil et al. J. Biol. Chem. 281 (2006) 3544-51). Flow cytometry analyses showed that R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. Moreover, R8-LP (PC-SOD) liposomes were found to scavenge superoxide anions (O-2^[-]) very efficiently. These results suggest that the efficient cytosolic delivery of PC-SOD by R8-modified liposomes would enhance the anti-oxidant effects of PC-SOD. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/44983 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山田 勇磨
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