Hokkaido University Collection of Scholarly and Academic Papers >
Faculty of Pharmaceutical Sciences >
Peer-reviewed Journal Articles, etc >
Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake
Title: | Octaarginine-modified liposomes enhance the anti-oxidant effect of Lecithinized superoxide dismutase by increasing its cellular uptake |
Authors: | Furukawa, Ryo Browse this author | Yamada, Yuma Browse this author | Takenaga, Mitsuko Browse this author | Igarashi, Rie Browse this author | Harashima, Hideyoshi Browse this author |
Keywords: | Lecithinized superoxide dismutase (PC-SOD) | Octaarginine-modified liposomes | Anti-oxidant therapy | Reactive oxygen species (ROS) | Mitochondria |
Issue Date: | 21-Jan-2011 |
Publisher: | Elsevier |
Journal Title: | Biochemical and Biophysical Research Communications |
Volume: | 404 |
Issue: | 3 |
Start Page: | 796 |
End Page: | 801 |
Publisher DOI: | 10.1016/j.bbrc.2010.12.062 |
PMID: | 21168389 |
Abstract: | The anti-oxidant enzyme superoxide dismutase (SOD) has the potential for use as a therapeutic agent in the treatment of various diseases caused by reactive oxygen species. However, achieving this would be difficult without a suitable delivery system for SOD. We previously reported that PC-SOD, in which four molecules of a phosphatidylcholine (PC) derivative were covalently bound to each dimer of recombinant human CuZnSOD, was a high affinity for the cell membrane (R. Igarashi et al, J. Pharmacol. Exp. Ther. 271 (1994) 1672-7). Here, we show that an octaarginine (R8) modified liposome equipped with PC-SOD (R8-LP (PC-SOD)) enhances its anti-oxidant effect. High-density R8-modified liposomes can stimulate macropinocytosis and are taken up efficiently by cells as demonstrated in a previous study (I.A. Khalil et al. J. Biol. Chem. 281 (2006) 3544-51). Flow cytometry analyses showed that R8-LP (PC-SOD) was taken up by cells more efficiently than PC-SOD. Moreover, R8-LP (PC-SOD) liposomes were found to scavenge superoxide anions (O-2^[-]) very efficiently. These results suggest that the efficient cytosolic delivery of PC-SOD by R8-modified liposomes would enhance the anti-oxidant effects of PC-SOD. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/44983 |
Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 山田 勇磨
|