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Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5

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Title: Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5
Authors: Yoshii, Kentaro Browse this author →KAKEN DB
Igarashi, Manabu Browse this author →KAKEN DB
Ito, Kimihito Browse this author →KAKEN DB
Kariwa, Hiroaki Browse this author →KAKEN DB
Holbrook, Michael R. Browse this author
Takashima, Ikuo Browse this author →KAKEN DB
Keywords: Omsk hemorrhagic fever
Infectious cDNA
Viral replication
Issue Date: Jan-2011
Publisher: Elsevier B.V.
Journal Title: Virus Research
Volume: 155
Issue: 1
Start Page: 61
End Page: 68
Publisher DOI: 10.1016/j.virusres.2010.08.023
PMID: 20817056
Abstract: Omsk hemorrhagic fever virus (OHFV) is a member of the tick-borne encephalitis serocomplex of flaviviruses, and causes hemorrhagic disease in humans. In this study, an infectious cDNA of OHFV was constructed to investigate the molecular mechanisms involved in OHFV pathogenesis for the first time. Our cDNA clone was capable of producing infectious virus which is genetically identical to the parental Guriev strain, and the recombinant virus showed similar biological properties to the parental virus including growth kinetics and virulence characteristics. While characterizing the cDNAs, fortuitous mutations at NS2A position 46 and NS5 position 836 were found to affect viral production. By using a viral replicon expressing luciferase, it was shown that both of the mutations produced a defect in RNA replication and that the NS5 mutation induced a temperature-sensitive phenotype, indicating the importance of these residues in RNA replication. This infectious cDNA will be a useful tool to study the replication and pathogenesis of OHFV.
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 好井 健太朗

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