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Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5
Title: | Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5 |
Authors: | Yoshii, Kentaro Browse this author →KAKEN DB | Igarashi, Manabu Browse this author →KAKEN DB | Ito, Kimihito Browse this author →KAKEN DB | Kariwa, Hiroaki Browse this author →KAKEN DB | Holbrook, Michael R. Browse this author | Takashima, Ikuo Browse this author →KAKEN DB |
Keywords: | Omsk hemorrhagic fever | Flavivirus | Infectious cDNA | Viral replication |
Issue Date: | Jan-2011 |
Publisher: | Elsevier B.V. |
Journal Title: | Virus Research |
Volume: | 155 |
Issue: | 1 |
Start Page: | 61 |
End Page: | 68 |
Publisher DOI: | 10.1016/j.virusres.2010.08.023 |
PMID: | 20817056 |
Abstract: | Omsk hemorrhagic fever virus (OHFV) is a member of the tick-borne encephalitis serocomplex of flaviviruses, and causes hemorrhagic disease in humans. In this study, an infectious cDNA of OHFV was constructed to investigate the molecular mechanisms involved in OHFV pathogenesis for the first time. Our cDNA clone was capable of producing infectious virus which is genetically identical to the parental Guriev strain, and the recombinant virus showed similar biological properties to the parental virus including growth kinetics and virulence characteristics. While characterizing the cDNAs, fortuitous mutations at NS2A position 46 and NS5 position 836 were found to affect viral production. By using a viral replicon expressing luciferase, it was shown that both of the mutations produced a defect in RNA replication and that the NS5 mutation induced a temperature-sensitive phenotype, indicating the importance of these residues in RNA replication. This infectious cDNA will be a useful tool to study the replication and pathogenesis of OHFV. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/45025 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 好井 健太朗
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