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Mitochondrial matrix delivery using MITO-Porter, a liposome-based carrier that specifies fusion with mitochondrial membranes
| Title: | Mitochondrial matrix delivery using MITO-Porter, a liposome-based carrier that specifies fusion with mitochondrial membranes |
| Authors: | Yasuzaki, Yukari Browse this author | | Yamada, Yuma Browse this author →KAKEN DB | | Harashima, Hideyoshi Browse this author |
| Keywords: | Mitochondria | | Mitochondrial matrix delivery | | Mitochondrial drug delivery | | Mitochondrial gene therapy | | MITO-Porter | | Membrane fusion | | mtDNA |
| Issue Date: | 25-Jun-2010 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 397 |
| Issue: | 2 |
| Start Page: | 181 |
| End Page: | 186 |
| Publisher DOI: | 10.1016/j.bbrc.2010.05.070 |
| PMID: | 20580633 |
| Abstract: | Mitochondria are the principal producers of energy in cells of higher organisms. It was recently reported that mutations and defects in mitochondrial DNA (mtDNA) are associated with various mitochondrial diseases including a variety of neurodegenerative and neuromuscular diseases. Therefore, an effective mitochondrial gene therapy and diagnosis would be expected to have great medical benefits. To achieve this, therapeutic agents need to be delivered into the innermost mitochondrial space (mitochondrial matrix), which contains the mtDNA pool. We previously reported on the development of MITO-Porter, a liposome-based carrier that introduces macromolecular cargos into mitochondria via membrane fusion. In this study, we provide a demonstration of mitochondrial matrix delivery and the visualization of mitochondrial genes (mtDNA) in living cells using the MITO-Porter. We first prepared MITO-Porter containing encapsulated propidium iodide (PI), a fluorescent dye used to stain nucleic acids to detect mtDNA. We then confirmed the emission of red-fluorescence from PI by conjugation with mtDNA, when the carriers were incubated in the presence of isolated rat liver mitochondria. Finally, intracellular observation by confocal laser scanning microscopy clearly verified that the MITO-Porter delivered PI to the mitochondrial matrix. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/45098 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山田 勇磨
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