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A layered structure of bacterial and archaeal communities and their in situ activities in anaerobic granules
| Title: | A layered structure of bacterial and archaeal communities and their in situ activities in anaerobic granules |
| Authors: | Satoh, Hisashi Browse this author | | Miura, Yuki Browse this author | | Tsushima, Ikuo Browse this author | | Okabe, Satoshi Browse this author |
| Keywords: | A layered structure in anaerobic granules | | anaerobic granules |
| Issue Date: | Nov-2007 |
| Publisher: | American Society for Microbiology |
| Journal Title: | Applied and Environmental Microbiology |
| Volume: | 73 |
| Issue: | 22 |
| Start Page: | 7300 |
| End Page: | 7307 |
| Publisher DOI: | 10.1128/AEM.01426-07 |
| Abstract: | The microbial community structure and spatial distribution of microorganisms and their in situ activities in anaerobic granules were investigated by 16S rRNA gene-based molecular techniques and microsensors for CH4, H2, pH, and oxidation-reduction potential (ORP). The 16S rRNA gene-cloning analysis revealed that the clones related to the phyla Alphaproteobacteria (detection frequency of 51%), Firmicutes (20%), Chloroflexi (9%), and Betaproteobacteria (8%) dominated the bacterial clone library and the predominant clones in the archaeal clone library were affiliated with Methanosaeta (73%). In situ hybridization with the oligonucleotide probes at the phylum level revealed that these microorganisms were numerically abundant in the granule. A layered structure of microorganisms was found in the granule, where Chloroflexi and Betaproteobacteria were present in the outer shell of the granule, Firmicutes was found in the middle layer, and aceticlastic Archaea was restricted to the inner layer. Microsensor measurements for CH4, H2, pH, and ORP revealed that acid and H2 production occurred in the upper part of the granule, below which H2 consumption and CH4 production were detected. Direct comparison of the in situ activity distribution with the spatial distribution of the microorganisms implied that Chloroflexi contributed to degradation of complex organic compounds in the outermost layer, H2 was produced mainly by Firmicutes in the middle layer, and Methanosaeta produced CH4 in the inner layer. We could also determine the effective diffusion coefficient for H2 in the anaerobic granules to be 2.66 × 10–5 cm2 s–1, which was 57% in water. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/45291 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐藤 久
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