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Use of Microelectrodes to Investigate the Effects of 2-chlorophenol on Microbial Activities in Biofilms

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/45293

Title: Use of Microelectrodes to Investigate the Effects of 2-chlorophenol on Microbial Activities in Biofilms
Authors: Satoh, Hisashi Browse this author
Sasaki, Yuichi Browse this author
Nakamura, Yoshiyuki Browse this author
Okabe, Satoshi Browse this author
Suzuki, Takuya Browse this author
Keywords: Inhibition of microbial activities
Biofilm
Microelectrodes
2-chlorophenol
Issue Date: Jul-2005
Publisher: Wiley-Blackwell
Journal Title: Biotechnology and Bioengineering
Volume: 91
Issue: 2
Start Page: 133
End Page: 138
Publisher DOI: 10.1002/bit.20506
Abstract: In order to assess the applicability of using microelectrodes as a tool for inhibition tests, temporal and spatial inhibitory effects of 2-chlorophenol (2-CP) on O2 respiration and nitrification activities in municipal wastewater biofilms were investigated using microelectrodes for O2 and NH4+. The time-course microelectrode measurements demonstrated that 2-CP inhibited O2 respiration and nitrification activities within 6 to 18 min. The microbial activities were inhibited only in the upper 400 µm of the biofilms by 2-CP, and the bacteria present in the deeper parts of the biofilms were still active, probably due to limited penetration of 2-CP. These results could reasonably explain the difference in inhibitory ratios of the O2 respiration and nitrification activities in the biofilms. O2 respiration activity was incompletely inhibited, which was attributed to the presence of O2 respiration activities in the deeper parts of the biofilm. In contrast, nitrification activity was significantly inhibited because ammonia-oxidizing bacteria were present in the upper parts of the biofilm. These results indicate that the microelectrodes with a very quick response time and a high spatial resolution are useful tools to study temporal and spatial inhibitory effects of inhibitors on in situ microbial activities in biofilms.
Rights: This is the pre-peer-reviewed version of the following article: Satoh, H., Sasaki, Y., Nakamura, Y., Okabe, S. and Suzuki, T. (2005), Use of microelectrodes to investigate the effects of 2-chlorophenol on microbial activities in biofilms. Biotechnology and Bioengineering, 91: 133–138. doi: 10.1002/bit.20506, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/bit.20506/abstract
Type: article (author version)
URI: http://hdl.handle.net/2115/45293
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐藤 久

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