Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes

Matsumoto, Ken'ichiro; Morimoto, Kenjiro; Gohda, Aoi; Shimada, Hiroaki; Taguchi, Seiichi

doi:10.1016/j.jbiosc.2010.11.020


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Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/45613

Title:	Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes
Other Titles:	翻訳効率の改善による組換え体タバコにおけるバイオプラスチック生産性の向上
Authors:	Matsumoto, Ken'ichiro Browse this author →KAKEN DB
	Morimoto, Kenjiro Browse this author
	Gohda, Aoi Browse this author
	Shimada, Hiroaki Browse this author
	Taguchi, Seiichi Browse this author →KAKEN DB
Keywords:	Transgenic plant
	Nicotiana tabacum
	PHA synthase
	Real-time PCR
Issue Date:	Apr-2011
Publisher:	Society for Biotechnology, Japan
Journal Title:	Journal of Bioscience and Bioengineering
Volume:	111
Issue:	4
Start Page:	485
End Page:	488
Publisher DOI:	10.1016/j.jbiosc.2010.11.020
PMID:	21185778
Abstract:	Polyhydroxybutyrate [P(3HB)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-CoA reductase (PhaB) and polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha (Cupriavidus necator) with optimized codon usage for expression in tobacco. P(3HB) contents in the transformants (0.2 mg/g dry cell weight in average) harboring the codon-optimized phaB gene was twofold higher than the control transformants harboring the wild-type phaB gene. The immunodetection revealed an increased production of PhaB in leaves, indicating that the enhanced expression of PhaB was effective to increase P(3HB) production in tobacco. In contrast, codon-optimization of the phaC gene exhibited no apparent effect on P(3HB) production. This result suggests the PhaB-catalyzed reaction to be a rate-determining step of P(3HB) biosynthesis in tobacco leaves.
Type:	article (author version)
URI:	http://hdl.handle.net/2115/45613
Appears in Collections:	工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松本謙一郎

OAI-PMH ( junii2 , jpcoar_1.0 )

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