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Elasticity of Living Cells on a Microarray during the Early Stages of Adhesion Measured by Atomic Force Microscopy

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Title: Elasticity of Living Cells on a Microarray during the Early Stages of Adhesion Measured by Atomic Force Microscopy
Authors: Mizutani, Yusuke Browse this author
Tsuchiya, Masahiro Browse this author
Hiratsuka, Shinichiro Browse this author
Kawahara, Koichi Browse this author →KAKEN DB
Tokumoto, Hiroshi Browse this author →KAKEN DB
Okajima, Takaharu Browse this author →KAKEN DB
Keywords: Atomic force microscope
Microarray
Fibroblast cells
Young's modulus
Log-normal distribution
Issue Date: Jul-2008
Publisher: Japan Society of Applied Physics
Journal Title: Japanese Journal of Applied Physics
Volume: 47
Issue: 7
Start Page: 6177
End Page: 6180
Publisher DOI: 10.1143/JJAP.47.6177
Abstract: The number distribution of the elastic modulus of fibroblast cells was successfully measured during the early stages of adhesion using an atomic force microscope (AFM) combined with a microarray as a substrate, which allowed us to arrange and culture cells so that a large number of cells could be measured in a short time period. We confirmed that the cells deposited in the wells of the microarray could be cultured for at least 12 h without any significant migration. Histograms of the Young's modulus, E, of the cells during the early stages of adhesion produced from force curve measurements of cells (n\cong300) cultured for 3–9 h were well fitted to a log-normal distribution function. With increasing incubation time, the average value of E increased significantly, while the standard deviation of the distribution remained almost constant. The results are discussed in terms of the cytoskeleton inside cells.
Relation: http://jjap.jsap.jp/link?JJAP/47/6177/
Type: article (author version)
URI: http://hdl.handle.net/2115/45746
Appears in Collections:情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岡嶋 孝治

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