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Hokkaido University Collection of Scholarly and Academic Papers >
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Elasticity of Living Cells on a Microarray during the Early Stages of Adhesion Measured by Atomic Force Microscopy
| Title: | Elasticity of Living Cells on a Microarray during the Early Stages of Adhesion Measured by Atomic Force Microscopy |
| Authors: | Mizutani, Yusuke Browse this author | | Tsuchiya, Masahiro Browse this author | | Hiratsuka, Shinichiro Browse this author | | Kawahara, Koichi Browse this author →KAKEN DB | | Tokumoto, Hiroshi Browse this author →KAKEN DB | | Okajima, Takaharu Browse this author →KAKEN DB |
| Keywords: | Atomic force microscope | | Microarray | | Fibroblast cells | | Young's modulus | | Log-normal distribution |
| Issue Date: | Jul-2008 |
| Publisher: | Japan Society of Applied Physics |
| Journal Title: | Japanese Journal of Applied Physics |
| Volume: | 47 |
| Issue: | 7 |
| Start Page: | 6177 |
| End Page: | 6180 |
| Publisher DOI: | 10.1143/JJAP.47.6177 |
| Abstract: | The number distribution of the elastic modulus of fibroblast cells was successfully measured during the early stages of adhesion using an atomic force microscope (AFM) combined with a microarray as a substrate, which allowed us to arrange and culture cells so that a large number of cells could be measured in a short time period. We confirmed that the cells deposited in the wells of the microarray could be cultured for at least 12 h without any significant migration. Histograms of the Young's modulus, E, of the cells during the early stages of adhesion produced from force curve measurements of cells (n\cong300) cultured for 3–9 h were well fitted to a log-normal distribution function. With increasing incubation time, the average value of E increased significantly, while the standard deviation of the distribution remained almost constant. The results are discussed in terms of the cytoskeleton inside cells. |
| Relation: | http://jjap.jsap.jp/link?JJAP/47/6177/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/45746 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 岡嶋 孝治
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