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Involvement of common intermediate 3-hydroxy-L-kynurenine in chromophore biosynthesis of quinomycin family antibiotics

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タイトル: Involvement of common intermediate 3-hydroxy-L-kynurenine in chromophore biosynthesis of quinomycin family antibiotics
著者: Hirose, Yuki 著作を一覧する
Watanabe, Kenji 著作を一覧する
Minami, Atsushi 著作を一覧する
Nakamura, Takemichi 著作を一覧する
Oguri, Hiroki 著作を一覧する
Oikawa, Hideaki 著作を一覧する
キーワード: biosynthesis
echinomycin
Streptomyces lasaliensis
Streptomyces Sp
SW-163D
発行日: 2011年 1月
出版者: Japan Antibiotics Research Association
誌名: Journal of Antibiotics
巻: 64
号: 1
開始ページ: 117
終了ページ: 122
出版社 DOI: 10.1038/ja.2010.142
抄録: Quinomycin antibiotics, represented by echinomycin, are an important class of antitumor antibiotics. We have recently succeeded in identification of biosynthetic gene clusters of echinomycin and SW-163D, and have achieved heterologous production of echinomycin in Escherichia coil. In addition, we have engineered echinomycin nonribosomal peptide synthetase (NRPS) to generate echinomycin derivatives. However, the biosynthetic pathways of intercalative chromophores quinoxaline-2-carboxylic acid (QXC) and 3-hydroxyquinaldic acid (HQA), which are important for biological activity, were not fully elucidated. Here, we report experiments involving incorporation of a putative advanced precursor, (2S, 3R)-[6'-2H]-3-hydroxy-L-kynurenine, and functional analysis of the enzymes Swb1 and Swb2 responsible for late-stage biosynthesis of HQA. Based on these experimental results, we propose biosynthetic pathways for both QXC and HQA via the common intermediate 3-hydroxy-L-kynurenine.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/46763
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 及川 英秋

 

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