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Visualization and motility of primordial germ cells using green fluorescent protein fused to 3'UTR of common carp nanos-related gene

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Title: Visualization and motility of primordial germ cells using green fluorescent protein fused to 3'UTR of common carp nanos-related gene
Authors: Kawakami, Yutaka Browse this author
Saito, Taiju Browse this author
Fujimoto, Takafumi Browse this author
Goto-Kazeto, Rie Browse this author
Takahashi, Eisuke Browse this author
Adachi, Shinji Browse this author →KAKEN DB
Arai, Katsutoshi Browse this author →KAKEN DB
Yamaha, Etsuro Browse this author →KAKEN DB
Keywords: nanos
primordial germ cell
common carp
goldfish
germline chimera
Issue Date: Jul-2011
Publisher: Elsevier
Journal Title: Aquaculture
Volume: 317
Issue: 1-4
Start Page: 245
End Page: 250
Publisher DOI: 10.1016/j.aquaculture.2011.04.019
Abstract: Primordial germ cells (PGCs) are the only cells in developing embryos with the potential to transmit genetic information to the next generation. We previously visualized the PGCs of several teleostean embryos by injecting RNA synthesized from constructs encoding green fluorescent protein (GFP) fused to the 3'UTR of the zebrafish (Danio rerio) nanos1 gene (nos1). However, this technique was not always suitable for visualizing PGCs in embryos from all teleost species. In this study, we compared the visualization of PGCs in common carp (Cyprinus carpio) embryos using two artificial constructs containing GFP fused to the 3'UTR of nanos from either common carp or zebrafish. Visualization was better using GFP fused to the 3'UTR of the nanos gene from common carp, compared with that from zebrafish. The visualized PGCs successfully migrated toward the gonadal ridge after transplantation into goldfish host embryos, suggesting that they maintained normal migratory motility. These techniques could be useful for the production of inter-specific germline chimeras using common carp donor PGCs.
Type: article (author version)
URI: http://hdl.handle.net/2115/46772
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川上 優

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