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Nuclear and cytoplasmic effects of human CRM1 on HIV-1 production in rat cells
| Title: | Nuclear and cytoplasmic effects of human CRM1 on HIV-1 production in rat cells |
| Authors: | Nagai-Fukataki, Mika Browse this author | | Ohashi, Takashi Browse this author | | Hashimoto, Iwao Browse this author | | Kimura, Tominori Browse this author | | Hakata, Yoshiyuki Browse this author | | Shida, Hisatoshi Browse this author |
| Issue Date: | Feb-2011 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Genes to Cells |
| Volume: | 16 |
| Issue: | 2 |
| Start Page: | 203 |
| End Page: | 216 |
| Publisher DOI: | 10.1111/j.1365-2443.2010.01476.x |
| PMID: | 21251165 |
| Abstract: | The human immunodeficiency virus type 1 (HIV-1) regulatory protein, Rev, mediates the nuclear export of unspliced gag and singly spliced env mRNAs by bridging viral RNA and the export receptor, CRM1. Recently, rat CRM1 was found to be less efficient than human CRM1 in supporting Rev function in rats. In this study, to understand the role of CRM1 in HIV propagation, the mechanism underlying the function of human and rat CRM1 in HIV-1 replication was investigated in rat cells. The production of viral particles, represented by the p24 Gag protein, was greatly enhanced by hCRM1 expression in rat cells; however, this effect was not simply due to the enhanced export of gag mRNA. The translation initiation rate of gag mRNA was not increased, nor was the Gag protein stabilized in the presence of hCRM1. However, the processing of the p55 Gag precursor and the release of viral particles were facilitated. These results indicated that hCRM1 exports gag mRNA to the cytoplasm, not only more efficiently than rCRM1 but also correctly, leading to efficient processing of Gag proteins and particle formation. |
| Rights: | The definitive version is available at wileyonlinelibrary.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/46874 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 志田 壽利
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