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Neuroprotective effect of a new DJ-1-binding compound against neurodegeneration in Parkinson's disease and stroke model rats

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タイトル: Neuroprotective effect of a new DJ-1-binding compound against neurodegeneration in Parkinson's disease and stroke model rats
著者: Kitamura, Yoshihisa 著作を一覧する
Watanabe, Shotaro 著作を一覧する
Taguchi, Masanobu 著作を一覧する
Takagi, Kentaro 著作を一覧する
Kawata, Takuya 著作を一覧する
Takahashi-Niki, Kazuko 著作を一覧する
Yasui, Hiroyuki 著作を一覧する
Maita, Hiroshi 著作を一覧する
Iguchi-Ariga, Sanae M. M. 著作を一覧する
Ariga, Hiroyoshi 著作を一覧する
発行日: 2011年 7月 8日
出版者: BioMed Central
誌名: Molecular Neurodegeneration
巻: 6
開始ページ: 48
出版社 DOI: 10.1186/1750-1326-6-48
抄録: Background: Parkinson's disease (PD) and cerebral ischemia are chronic and acute neurodegenerative diseases, respectively, and onsets of these diseases are thought to be induced at least by oxidative stress. PD is caused by decreased dopamine levels in the substantia nigra and striatum, and cerebral ischemia occurs as a result of local reduction or arrest of blood supply. Although a precursor of dopamine and inhibitors of dopamine degradation have been used for PD therapy and an anti-oxidant have been used for cerebral ischemia therapy, cell death progresses during treatment. Reagents that prevent oxidative stress-induced cell death are therefore necessary for fundamental therapies for PD and cerebral ischemia. DJ-1, a causative gene product of a familial form of PD, PARK7, plays roles in transcriptional regulation and anti-oxidative stress, and loss of its function is thought to result in the onset of PD. Superfluous oxidation of cysteine at amino acid 106 (C106) of DJ-1 renders DJ-1 inactive, and such oxidized DJ-1 has been observed in patients with the sporadic form of PD. Results: In this study, a compound, comp-23, that binds to DJ-1 was isolated by virtual screening. Comp-23 prevented oxidative stress-induced death of SH-SY5Y cells and primary neuronal cells of the ventral mesencephalon but not that of DJ-1-knockdown SH-SY5Y cells, indicating that the effect of the compound is specific to DJ-1. Comp-23 inhibited the production of reactive oxygen species (ROS) induced by oxidative stress and prevented excess oxidation of DJ-1. Furthermore, comp-23 prevented dopaminergic cell death in the substantia nigra and restored movement abnormality in 6-hydroxyldopamine-injected and rotenone-treated PD model rats and mice. Comp-23 also reduced infarct size of cerebral ischemia in rats that had been induced by middle cerebral artery occlusion. Protective activity of comp-23 seemed to be stronger than that of previously identified compound B. Conclusions: The results indicate that comp-23 exerts a neuroprotective effect by reducing ROS-mediated neuronal injury, suggesting that comp-23 becomes a lead compound for PD and ischemic neurodegeneration therapies.
資料タイプ: article
URI: http://hdl.handle.net/2115/46905
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 有賀 寛芳

 

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