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Sphingolipid synthesis is involved in autophagy in Saccharomyces cerevisiae

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Title: Sphingolipid synthesis is involved in autophagy in Saccharomyces cerevisiae
Authors: Yamagata, Maki Browse this author
Obara, Keisuke Browse this author
Kihara, Akio Browse this author
Keywords: Autophagy
Issue Date: 15-Jul-2011
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 410
Issue: 4
Start Page: 786
End Page: 791
Publisher DOI: 10.1016/j.bbrc.2011.06.061
PMID: 21703229
Abstract: In eukaryotes, autophagy is a conserved protein degradation system that degrades cytoplasmic components by encompassing them with double-membrane structures, called autophagosomes, and delivering them to the lytic compartments of vacuoles/lysosomes. Certain Atg proteins are known to be involved in autophagy, yet the identity and function of lipid molecules involved remain largely unknown. We investigated the involvement of sphingolipids in autophagy using Saccharomyces cerevisiae. Inhibiting synthesis of the simplest complex sphingolipid, inositol phosphorylceramide (IPC), resulted in reduced autophagic activities. Similar results were obtained using myriocin, an inhibitor of the first step in sphingolipid synthesis. Our results indicate that sphingolipids, especially IPC, are required for autophagy. Inhibition of sphingolipid synthesis had no effect on formation of Atg12-Atg5 or Atg8-phosphatidylethanolamine conjugates, on maturation of vacuolar proteases, or on formation of the pre-autophagosomal structure (PAS). These results suggest that sphingolipids are not involved in the cellular signaling that leads to formation of the PAS, but may be involved in the process of autophagosome formation.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木原 章雄

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