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Formation of 5-formyl-2'-deoxycytidine from 5-methyl-2'-deoxycytidine in duplex DNA by Fenton-type reactions and gamma-irradiation
| Title: | Formation of 5-formyl-2'-deoxycytidine from 5-methyl-2'-deoxycytidine in duplex DNA by Fenton-type reactions and gamma-irradiation |
| Authors: | Murata-Kamiya, Naoko Browse this author | | Kamiya, Hiroyuki Browse this author | | Karino, Naoko Browse this author | | Ueno, Yoshihito Browse this author | | Kaji, Hiroshi Browse this author | | Matsuda, Akira Browse this author | | Kasai, Hiroshi Browse this author |
| Issue Date: | Nov-1999 |
| Publisher: | Oxford University Press |
| Journal Title: | Nucleic Acids Research |
| Volume: | 27 |
| Issue: | 22 |
| Start Page: | 4385 |
| End Page: | 4390 |
| Publisher DOI: | 10.1093/nar/27.22.4385 |
| Abstract: | 5-Methyl-2′-deoxycytidine (5-Me-dC) is formed by the enzymatic methylation of dC, primarily in CpG sequences in DNA, and is involved in the regulation of gene expression. In the present study, 5-Me-dC and double-stranded DNA fragments containing 5-Me-dC were either γirradiated or aerobically treated with Fenton-type reagents, Fe(ll)-EDTA, Fe(ll)-nitrilotri-acetic acid, Fe(lll)-EDTA-H2O2-catechol or ascorbic acid-H2O2 under neutral conditions. The formation of 5-formyl-2′-deoxycytidine (5-CHO-dC) was observed upon treatment of both 5-Me-dC and DNA fragments containing 5-Me-dC. The yields of 5-CHO-dC from 5-Me-dC and those of 5-formyl-2′-deoxyuridine from dT were comparable. These results suggest that 5-Me-dC in DNA is as susceptible to oxidation as dT in cells, and raise the possibility that 5-CHO-dC may contribute to the high mutagenic rate observed in CpG sequences in genomic DNA. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/46962 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 彰
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