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Site-specific introduction of functional groups into phosphodiester oligodeoxynucleotides and their thermal stability and nuclease- resistance properties
| Title: | Site-specific introduction of functional groups into phosphodiester oligodeoxynucleotides and their thermal stability and nuclease- resistance properties |
| Authors: | Nomura, Yukari Browse this author | | Ueno, Yoshihito Browse this author | | Matsuda, Akira Browse this author |
| Issue Date: | Jul-1997 |
| Publisher: | Oxford University Press |
| Journal Title: | Nucleic Acids Research |
| Volume: | 25 |
| Issue: | 14 |
| Start Page: | 2784 |
| End Page: | 2791 |
| Publisher DOI: | 10.1093/nar/25.14.2784 |
| Abstract: | We report here the site-specific introduction of functional groups into phosphodiester oligodeoxynucleotides (ODNs). ODNs containing both 5-(N-aminohexyl)-carbamoyl-2′-deoxyuridine (H), which serves as a tether for the further conjugation of functional groups, and 5-(N,N-dimethylaminohexyl)carbamoyl-2′-deoxyuridine (D), which contributes to the thermal stability of the duplex and to the resistance to nucleolytic hydrolysis by nucleases, were synthesized. Functional groups such as folic acid and palmitic acid were site-specifically introduced into the terminus of the aminohexyl-linker of H. The thermal stability and resistance toward nuclease digestion of the modified ODNs were studied. We found that ODNs containing D and H formed stable duplexes with both the complementary DNA and RNA strands even when a bulky functional group such as folic acid, palmitic acid or cholesterol was attached to the terminus of the amino-linker. We also found that ODN analogues which contained D were more resistant to nucleolytic degradation by exo- and endonuclease than the unmodified ODN. Furthermore, duplexes formed by ODNs containing D and the complementary RNA could elicit RNase H activity. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/46967 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 彰
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