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Dynamic Behavior and Spontaneous Differentiation of Mouse Embryoid Bodies on Hydrogel Substrates of Different Surface Charge and Chemical Structures

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Title: Dynamic Behavior and Spontaneous Differentiation of Mouse Embryoid Bodies on Hydrogel Substrates of Different Surface Charge and Chemical Structures
Authors: Liu, Jian Fang Browse this author
Chen, Yong Mei Browse this author
Yang, Jing Jing Browse this author
Kurokawa, Takayuki Browse this author →KAKEN DB
Kakugo, Akira Browse this author →KAKEN DB
Yamamoto, Kimiko Browse this author
Gong, Jian Ping Browse this author →KAKEN DB
Issue Date: 25-Aug-2011
Publisher: Mary Ann Liebert
Journal Title: Tissue Engineering Part A
Volume: 17
Issue: 17-18
Start Page: 2343
End Page: 2357
Publisher DOI: 10.1089/ten.tea.2011.0034
PMID: 21548714
Abstract: Differentiation of embryoid bodies (EBs) into particular cell lineages has been extensively studied. There is anincreasing interest in the effect of soft hydrogel scaffolds on the behavior of EBs, such as the initial adhesion,dynamic morphology change, and differentiation. In this study, without adding any other bioactive factors inthe serum-containing medium, dynamic behaviors of mouse EBs loaded on the surface of hydrogels withdifferent surface charge and chemical structures are investigated. EBs adhered quickly to negatively chargedpoly(sodium p-styrene sulfonate) (PNaSS) hydrogels, which facilitates EBs spreading, migration, and differentiationinto three germ layers with high efficiency of cardiomyocytes differentiation, similar to that on gelatincoated polystyrene (PS) culture plate. While on neutral poly(acrylamide) (PAAm) hydrogels, EBs maintained theinitial spherical morphology with high expression of pluripotency-related markers in the short culture periods,and then showed the significantly greater levels of selected endoderm markers after long-time culture. EBscultured on negatively charged poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS) gelsdemonstrated the analogous behaviors with that of neutral PAAm gels at early differentiation phase (day 4 + 1).Then, their adhesion, spreading and differentiation were quite similar to that on negatively charged PNaSS gels.The correlation between surface properties of hydrogels and EBs differentiation was discussed.
Rights: This is a copy of an article published in the TISSUE ENGINEERING: Part A © 2011 Mary Ann Liebert, Inc.; TISSUE ENGINEERING: Part A is available online at:
Type: article
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 龔 剣萍 (Gong Jian Ping)

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