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Significance of radical oxygen production in sorus development and zoospore germination in Saccharina japonica (Phaeophyceae)

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Title: Significance of radical oxygen production in sorus development and zoospore germination in Saccharina japonica (Phaeophyceae)
Authors: Mizuta, Hiroyuki Browse this author →KAKEN DB
Yasui, Hajime Browse this author →KAKEN DB
Keywords: antioxidant
reactive oxygen
zoospore germination
Issue Date: Oct-2010
Publisher: Walter de Gruyter
Journal Title: Botanica Marina
Volume: 53
Issue: 5
Start Page: 409
End Page: 416
Publisher DOI: 10.1515/BOT.2010.047
Abstract: We investigated production of radical oxygen species (ROS) in sorus development of sporophyte discs and during zoospore germination of Saccharina japonica (Phaeophyceae) using the fluorescent dye 2',7'-dichlorofluorescein diacetate (DCFH-DA). Quantitative analysis of ROS showed high intracellular production during the stages of zoosporangium formation when paraphyses elongated. In this stage, remarkable ROS release was observed. ROS production was also observed histologically (under a fluorescence microscope) in the elongating paraphyses and the sorus zoosporangia of the sorus. The sorus had significantly higher phenol content, anti-oxidant capacity and ROS scavenging enzyme activities (ascorbate peroxidase, catalase, glutathione reductase and superoxide dismutase) than adjacent non-sorus blade sectors. Thus, intracellular ROS scavenging mechanisms are active during ROS production in sorus formation. In contrast, iodine content was lower in the sorus than in the adjacent non-sorus tissues, suggesting the existence of an anti-oxidant defense mechanism based on iodine efflux. We demonstrated that ROS production is an important phenomenon in the reproduction of the sporophyte in S. japonica. Furthermore, ROS were also observed in the zoospore germination processes. We discuss the physiological and ecological roles of ROS production in sorus formation and zoospore germination.
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 水田 浩之

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