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Hokkaido University Collection of Scholarly and Academic Papers >
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Significance of radical oxygen production in sorus development and zoospore germination in Saccharina japonica (Phaeophyceae)
| Title: | Significance of radical oxygen production in sorus development and zoospore germination in Saccharina japonica (Phaeophyceae) |
| Authors: | Mizuta, Hiroyuki Browse this author →KAKEN DB | | Yasui, Hajime Browse this author →KAKEN DB |
| Keywords: | antioxidant | | reactive oxygen | | Saccharina | | sorus | | zoospore germination |
| Issue Date: | Oct-2010 |
| Publisher: | Walter de Gruyter |
| Journal Title: | Botanica Marina |
| Volume: | 53 |
| Issue: | 5 |
| Start Page: | 409 |
| End Page: | 416 |
| Publisher DOI: | 10.1515/BOT.2010.047 |
| Abstract: | We investigated production of radical oxygen species (ROS) in sorus development of sporophyte discs and during zoospore germination of Saccharina japonica (Phaeophyceae) using the fluorescent dye 2',7'-dichlorofluorescein diacetate (DCFH-DA). Quantitative analysis of ROS showed high intracellular production during the stages of zoosporangium formation when paraphyses elongated. In this stage, remarkable ROS release was observed. ROS production was also observed histologically (under a fluorescence microscope) in the elongating paraphyses and the sorus zoosporangia of the sorus. The sorus had significantly higher phenol content, anti-oxidant capacity and ROS scavenging enzyme activities (ascorbate peroxidase, catalase, glutathione reductase and superoxide dismutase) than adjacent non-sorus blade sectors. Thus, intracellular ROS scavenging mechanisms are active during ROS production in sorus formation. In contrast, iodine content was lower in the sorus than in the adjacent non-sorus tissues, suggesting the existence of an anti-oxidant defense mechanism based on iodine efflux. We demonstrated that ROS production is an important phenomenon in the reproduction of the sporophyte in S. japonica. Furthermore, ROS were also observed in the zoospore germination processes. We discuss the physiological and ecological roles of ROS production in sorus formation and zoospore germination. |
| Relation: | http://www.degruyter.com/journals/bm/detailEn.cfm?sel=pr |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/47108 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 水田 浩之
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