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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Cloning and functional characterization of Chondrichthyes, cloudy catshark, Scyliorhinus torazame and whale shark, Rhincodon typus estrogen receptors
| Title: | Cloning and functional characterization of Chondrichthyes, cloudy catshark, Scyliorhinus torazame and whale shark, Rhincodon typus estrogen receptors |
| Authors: | Katsu, Yoshinao Browse this author →KAKEN DB | | Kohno, Satomi Browse this author | | Narita, Haruka Browse this author | | Urushitani, Hiroshi Browse this author | | Yamane, Koudai Browse this author | | Hara, Akihiko Browse this author | | Clauss, Tonya M. Browse this author | | Walsh, Michael T. Browse this author | | Miyagawa, Shinichi Browse this author | | Guillette, Louis J. Browse this author | | Iguchi, Taisen Browse this author |
| Keywords: | shark | | estrogen receptor | | transactivation | | evolution | | environmental chemicals |
| Issue Date: | 15-Sep-2010 |
| Publisher: | Elsevier |
| Journal Title: | General and Comparative Endocrinology |
| Volume: | 168 |
| Issue: | 3 |
| Start Page: | 496 |
| End Page: | 504 |
| Publisher DOI: | 10.1016/j.ygcen.2010.06.010 |
| Abstract: | Sex-steroid hormones are essential for normal reproductive activity in both sexes in all vertebrates. Estrogens are required for ovarian differentiation during a critical developmental stage and promote the growth and differentiation of the female reproductive system following puberty. Recent studies have shown that environmental estrogens influence the developing reproductive system as well as gametogenesis, especially in males. To understand the molecular mechanisms of estrogen actions and to evaluate estrogen receptor ligand interactions in Elasmobranchii, we cloned a single estrogen receptor (ESR) from two shark species, the cloudy catshark (Scyliorhinus torazame) and whale shark (Rhincodon typus) and used an ERE-luciferase reporter assay system to characterize the interaction of these receptors with steroidal and other environmental estrogens. In the transient transfection ERE-luciferase reporter assay system, both shark ESR proteins displayed estrogen-dependent activation of transcription, and shark ESRs were more sensitive to 17β-estradiol compared with other natural and synthetic estrogens. Further, the environmental chemicals, bisphenol A, nonylphenol, octylphenol and DDT could activate both shark ESRs. The assay system provides a tool for future studies examining the receptor-ligand interactions and estrogen disrupting mechanisms in Elasmobranchii. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/47112 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 勝 義直
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